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PMID: 6253923 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interaction of the cAMP receptor protein with the lac promoter.

Nucleic acids research ·Vol. 8 ·No. 4 ·1980-02-25 ·Pages 759-66

Simpson RB

Abstract

The cyclic AMP receptor protein (CRP) stimulates transcription of the lactose operon by binding to the lac promoter. I have identified those 5-positions of thymines in the promoter that lie close to bound CRP. Although ultraviolet irradiation of DNA with 5-bromouracil substituted in place of thymine normally cleaves the DNA at the bromouracils, a protein bound to the DNA can perturb these cleavages at those locations at which the protein lies close to the bromine. The contacts inferred from this photochemical probe and the results of nucleolytic attack of this complex by exonuclease III support a model where the cyclic AMP receptor protein binds to the promoter making symmetrical contacts with one face of the double helix and then stimulates transcription through contacts with RNA polymerase.

MeSH Terms
Binding Sites Bromouracil DNA Restriction Enzymes DNA-Directed RNA Polymerases/metabolism Escherichia coli/metabolism Lac Operon Models, Genetic Photochemistry Receptors, Cyclic AMP/metabolism Transcription, Genetic
Chemicals
Receptors, Cyclic AMP Bromouracil DNA-Directed RNA Polymerases DNA Restriction Enzymes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Simpson R B
References (20)
20 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1980-02-25
Pages
759-66
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327308
Subset
IM
Grants
NIGMS NIH HHS · GM--21514 · United States
External Links
PubMed source PMC Full Text View journal details
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