Enzyme-linked immunosorbent assay for detection of antibody in volunteers experimentally infected with human coronavirus strain 229 E.

Journal of clinical microbiology ·Vol. 12 ·No. 4 ·1980-10-00 ·Pages 493-7

Kraaijeveld CA, Reed SE, Macnaughton MR

Abstract

An enzyme-linked immunosorbent assay was developed for detecting antibody rises to human coronavirus strain 229E and related strains in paired sera from infected volunteers. There was a close correlation between development of colds infected volunteers. There was a close correlation between development of colds and significant antibody rises detected by the enzyme-linked immunosorbent assay. Furthermore, the assay was more sensitive than a neutralization assay. This enzyme-linked immunosorbent assay is an easy, accurate, and sensitive assay for measuring significant antibody rises to human coronavirus strain 229E group viruses, and it could be useful in the clinical diagnosis of these infections.

MeSH Terms

Antibodies, Viral/analysis Antigens, Viral Coronaviridae/immunology Coronaviridae Infections/immunology Enzyme-Linked Immunosorbent Assay Humans

Chemicals

Antibodies, Viral Antigens, Viral

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kraaijeveld C A

Reed S E

Macnaughton M R

References (14)

14 references, click to expand

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.12.4.493-497.1980

Published

1980-10-00

Pages

493-7

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC273622

Subset

External Links

PubMed source DOI: 10.1128/jcm.12.4.493-497.1980 PMC Full Text View journal details

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