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PMID: 6252190 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Salmonella typhimurium mutants with reduced levels of transfer ribonucleic acid-inhibitable endodeoxyribonucleolytic activity.

Journal of bacteriology ·Vol. 144 ·No. 1 ·1980-10-00 ·Pages 173-8

Zabel DJ, Trucksis M, Depew RE

Abstract

Two methods of mutagenesis, chemical alkylation and insertion of the tetracycline resistance transposon Tn10, were used to generate mutants of Salmonella typhimurium which had reduced levels of endodeoxyribonucleolytic activity. The chemically induced mutations defined a locus, endA, which was cotransduced with serA at a low frequency and with metK at a high frequency. Three-factor crosses revealed that metK was between serA and endA. The major endodeoxyribonucleolytic activity in crude extracts of s. typhimurium was similar to the activity of Escherichia coli endonuclease I. A Tn10 insertion mutation of endA resulted in the most severe loss of endodeoxyribonucleolytic activity among the endA alleles studied. Two of the chemically induced mutations resulted in activities which were more thermolabile than the wild-type activity.

MeSH Terms
DNA Transposable Elements DNA, Bacterial/metabolism Endonucleases/genetics,metabolism Genes Hot Temperature Mutation RNA, Transfer/pharmacology Salmonella typhimurium/enzymology,genetics
Chemicals
DNA Transposable Elements DNA, Bacterial RNA, Transfer Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zabel D J
Trucksis M
Depew R E
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21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.144.1.173-178.1980
Published
1980-10-00
Pages
173-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC294614
Subset
IM
Grants
NIGMS NIH HHS · GM25500 · United States
External Links
PubMed source DOI: 10.1128/jb.144.1.173-178.1980 PMC Full Text View journal details
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