Antiviral activity of tunicamycin on herpes simplex virus.

Antimicrobial agents and chemotherapy ·Vol. 17 ·No. 6 ·1980-06-00 ·Pages 1014-22

Katz E, Margalith E, Duksin D

Abstract

Tunicamycin (0.5 microgram/ml) significantly lowers (2 to 3 log10) the infectious yield of herpes simplex virus type 1 grown in chicken embryo fibroblasts and in BSC1 cells. Although virus particles are formed and the synthesis of the viral deoxyribonucleic acid is only partially affected by the antibiotic, the glycosylation of herpesvirus glycopeptides is amost completely inhibited. The morphology of virus particles made in the presence of tunicamycin is similar to that of intact virus particles, as demonstrated by electron microscopy. This suggests that the absence of the carbohydrate side-chain from the viral glycopeptides does not affect the overall integrity of the virion but decreases very significantly the infectivity of these particles.

MeSH Terms

DNA, Viral/biosynthesis Electrophoresis, Polyacrylamide Gel Glucosamine/analogs & derivatives Glycopeptides/biosynthesis,physiology Microscopy, Electron Simplexvirus/drug effects,growth & development,metabolism,ultrastructure Thymidine/metabolism Time Factors Tunicamycin/pharmacology Viral Proteins/biosynthesis

Chemicals

DNA, Viral Glycopeptides Viral Proteins Tunicamycin Glucosamine Thymidine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Katz E

Margalith E

Duksin D

References (26)

26 references, click to expand

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Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.17.6.1014

Published

1980-06-00

Pages

1014-22

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC283921

Subset

External Links

PubMed source DOI: 10.1128/AAC.17.6.1014 PMC Full Text View journal details

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Antiviral activity of tunicamycin on herpes simplex virus.

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