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PMID: 6248722 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genetics and physiology of the rel system of Bacillus subtilis.

Molecular & general genetics : MGG ·Vol. 178 ·No. 2 ·1980-00-00 ·Pages 271-9

Smith I, Paress P, Cabane K, Dubnau E

Abstract

Stringent factor (ATP:GTP-3' pyrophosphotransferase) has been purified from wild type Bacillus subtilis and it has been shown that guanosine tetra- and pentaphosphate (ppGpp and pppGpp) are synthesized in vitro in the presence of ribosomes, unacylated tRNA and its specific codon, as has been demonstrated in Escherichia coli. relA, the genetic determinant for the stringent factor, has been mapped on the B. subtilis chromosome by transduction and is found between aroD and leu. The relC locus, defined by mutations which were originally selected by resistance to thiostrepton, has been mapped adjacent to spoOH in the order cysA, spoOH, relC, rif. Sringent factor and ribosomes are functional for the in vitro synthesis of (p)ppGpp in early stages of sporulation (up to at least 4 h). This contradicts the findings of other laboratories.

MeSH Terms
Bacillus subtilis/genetics,physiology Chromosome Mapping Chromosomes, Bacterial/ultrastructure GTP Pyrophosphokinase/genetics Genes Mutation Phosphotransferases/genetics Spores, Bacterial Transduction, Genetic
Chemicals
Phosphotransferases GTP Pyrophosphokinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Smith I
Paress P
Cabane K
Dubnau E
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38 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00270472
Published
1980-00-00
Pages
271-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00270472
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