Electron microscopic mapping and sequence analysis of the terminator for the early message of E. coli phage T7.

Molecular & general genetics : MGG ·Vol. 178 ·No. 1 ·1980-04-00 ·Pages 27-34

Priess H, Koller B, Hess B, Delius H

Abstract

The terminator position of T7 early messenger RNA was determined by electron microscopic measurements. The end of the RNA was mapped at a position 18.9% from the left end of T7 DNA, and 145 +/- 25 nucleotides from the right end of the Hpa I fragment Q. The sequence of the Hpa I Q fragment was determined around this position, and a terminator-like structure was detected in position 193 to 169 from the right end of fragment Q.

MeSH Terms

Base Sequence Chromosome Mapping DNA Restriction Enzymes/metabolism DNA, Viral/genetics Escherichia coli/genetics Microscopy, Electron Nucleic Acid Conformation RNA, Messenger/genetics T-Phages/genetics

Chemicals

DNA, Viral RNA, Messenger DNA Restriction Enzymes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Priess H

Koller B

Hess B

Delius H

References (29)

29 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00267209

Published

1980-04-00

Pages

27-34

Language

English

Region

Germany

NLM ID

0125036

Subset

Databases

GENBANK

J02518

External Links

PubMed source DOI: 10.1007/BF00267209

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Electron microscopic mapping and sequence analysis of the terminator for the early message of E. coli phage T7.

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