Identification of a positive regulatory protein in Escherichia coli: the product of the cysB gene.

Molecular & general genetics : MGG ·Vol. 177 ·No. 3 ·1980-02-00 ·Pages 535-9

Mascarenhas DM, Yudkin MD

Abstract

From the specialized transducing bacteriophage lambdacysB, recombinant phages lambdacysB242 and lambdacysB257 have been obtained, each of which carries an amber mutation in the cysB cistron. A comparison of polyacrylamide gel electrophoretic profiles of labelled extracts from uv-irradiated bacteria that had been infected with lambdacysB+ or with lambdacysB-amber phages, led to the identification of a 39,000-dalton polypeptide as the product of the cysB gene. The native protein was purified to near radiochemical purity and was found to be an oligomer with an isoelectric point close to pH 7.

MeSH Terms

Bacterial Proteins/genetics,isolation & purification Bacteriophage lambda/genetics Cysteine/genetics Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics Genes Genetic Vectors Protein Biosynthesis

Chemicals

Bacterial Proteins Cysteine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Mascarenhas D M

Yudkin M D

References (14)

14 references, click to expand

Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Characterization of lambdatrp-transducing bacteriophages made in vitro.
J Mol Biol. 1976 Nov 15;107(4):549-69 PMID: 794488
Reversible denaturation of enzymes by sodium dodecyl sulfate.
J Biol Chem. 1971 Jul 25;246(14):4504-9 PMID: 5106387
Mutations in Escherichia coli that relieve catabolite repression of tryptophanase synthesis. Mutations distant from the tryptophanase gene.
J Gen Microbiol. 1976 Jan;92(1):125-32 PMID: 173793
S-amino acid metabolism and its regulation in Escherichia coli and Salmonella typhimurium.
Adv Genet. 1971;16:141-65 PMID: 4947102
Fine-structure mapping and complementation analysis of the Escherichia coli cysB gene.
J Bacteriol. 1977 Jul;131(1):49-56 PMID: 326769
The construction in vitro of transducing derivatives of phage lambda.
Mol Gen Genet. 1976 Jul 23;146(2):199-207 PMID: 785220
The nature of the product of the cys B gene of Escherichia coli.
Mol Gen Genet. 1975;136(2):181-3 PMID: 16094983
Polarity suppressors increase expression of the wild-type tryptophan operon of Escherichia coli.
J Mol Biol. 1976 May 15;103(2):395-409 PMID: 781273
Analysis of merodiploids of the cysB region in Salmonella typhimurium.
Mol Gen Genet. 1978 Sep 20;165(1):31-8 PMID: 362164
Isolation of a lambdadcys transducing bacteriophage and its use in determining the regulation of cysteine messenger ribonucleic acid synthesis in Escherichia coli K-12.
J Bacteriol. 1977 Dec;132(3):757-63 PMID: 336603
Genetic and biochemical properties of an association complex between host components and lambda DNA.
Virology. 1973 Mar;52(1):105-19 PMID: 4610985
Positive control of sulphate reduction in Escherichia coli. The nature of the pleiotropic cysteineless mutants of E. coli K12.
Biochem J. 1968 Dec;110(3):597-602 PMID: 4882982
Regulation of L-cysteine biosynthesis in Salmonella typhimurium. I. Effects of growth of varying sulfur sources and O-acetyl-L-serine on gene expression.
J Biol Chem. 1971 Jun 10;246(11):3474-84 PMID: 4931306

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00271494

Published

1980-02-00

Pages

535-9

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00271494

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Identification of a positive regulatory protein in Escherichia coli: the product of the cysB gene.

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