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PMID: 6245072 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Physiological function of periplasmic hexose phosphatase in Salmonella typhimurium.

Journal of bacteriology ·Vol. 141 ·No. 3 ·1980-03-00 ·Pages 1474-7

Rephaeli AW, Artenstein IR, Saier MH

Abstract

Hydrolysis of sugar phosphates by crude and purified preparations of periplasmic hexose phosphatase from Salmonella typhimurium followed Michaelis-Menten kinetics. The enzyme bound glucose 1-phosphate with high affinity (Km = 10 microM) but bound glucose 6-phosphate with low affinity (Km = 2,000 microM). The order of substrate affinities was glucose 1-phosphate greater than mannose 1-phosphate = galactose 1-phosphate greater than fructose 1-phosphate greater than glucose 6-phosphate. These results and others suggest that the physiological function of the enzyme is the periplasmic hydrolysis of hexose 1-phosphates.

MeSH Terms
Cytoplasm/enzymology Fructosephosphates/metabolism Galactosephosphates/metabolism Glucosephosphates/metabolism Kinetics Mannosephosphates/metabolism Phosphoric Monoester Hydrolases/metabolism Salmonella typhimurium/enzymology Substrate Specificity
Chemicals
Fructosephosphates Galactosephosphates Glucosephosphates Mannosephosphates hexose phosphatase Phosphoric Monoester Hydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rephaeli A W
Artenstein I R
Saier M H
References (9)
9 references, click to expand
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  5. Isolation and characterization of mutations affecting the transport of hexose phosphates in Escherichia coli.
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    J Bacteriol. 1976 Jul;127(1):120-7 PMID: 179973
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    J Bacteriol. 1977 Apr;130(1):399-410 PMID: 192712
  8. Regulation of two phosphatases and a cyclic phosphodiesterase of Salmonella typhimurium.
    J Bacteriol. 1977 Apr;130(1):420-8 PMID: 192713
  9. Kinetic analyses of the sugar phosphate:sugar transphosphorylation reaction catalyzed by the glucose enzyme II complex of the bacterial phosphotransferase system.
    J Biol Chem. 1978 Nov 10;253(21):7595-7 PMID: 359550
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.141.3.1474-1477.1980
Published
1980-03-00
Pages
1474-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC293865
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.141.3.1474-1477.1980 PMC Full Text View journal details
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