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PMID: 6244575 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Autoradiographic localization of gamma-aminobutyric acid (GABA) receptors in the rat cerebellum.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 1 ·1980-01-00 ·Pages 670-4

Palacios JM, Young WS, Kuhar MJ

Abstract

[3H]Muscimol was used in the light microscopic autoradiographic localization of gamma-aminobutyric acid (GABA) receptors in the rat cerebellum. The binding of [3H]-muscimol to the slide-mounted tissue sections had all of the characteristics associated with a GABA receptor. It was saturable with appropriate kinetic constants and was blocked only by other GABAergic drugs. Autoradiographic studies revealed a very high density of receptors over the granule cell layer, a low level over the molecular layer, and negligible receptor binding over white matter. This suggested a high localization of GABA receptors to granule cells, which is supported by other neurochemical studies. The autoradiographic procedure used here should be a useful method in further studies of receptors requiring high sensitivity and increased anatomical resolution.

MeSH Terms
Animals Autoradiography Cerebellum/metabolism Kinetics Male Muscimol/metabolism Rats Receptors, Neurotransmitter/metabolism gamma-Aminobutyric Acid/metabolism
Chemicals
Receptors, Neurotransmitter Muscimol gamma-Aminobutyric Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Palacios J M
Young W S
Kuhar M J
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.1.670
Published
1980-01-00
Pages
670-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348337
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.1.670 PMC Full Text View journal details
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