Target cell specificity of defective avian leukemia viruses: hematopoietic target cells for a given virus type can be infected but not transformed by strains of a different type.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 1 ·1980-01-00 ·Pages 389-93

Graf T, Beug H, Hayman MJ

Abstract

Defective avian leukemia viruses of the avian erythroblastosis (AEV), avian myelocytomatosis (MC29), and avian myeloblastosis (AMV) type induce the proliferation of leukemic cells with properties of erythroblasts, macrophages, and myeloblasts, respectively. Their target cells can be separated and have properties of cells of the erythroid (AEV) and myeloid lineage (MC29 and AMV), respectively. In the present study we have shown that this target cell specificity is not due to the ability of the different strains to infect only certain types of hematopoietic cells. Instead, AEV was found to replicate in macrophages and to induce the expression of p75 AEV, its presumptive transforming protein. Likewise, MC29 was found to replicate in AEV-infected erythroblasts as well as in AMV-infected myeloblasts and to express the p110 MC29 protein in these cells. Superinfection with MC29 or AMV of ts34 AEV-infected erythroblasts did not impair their capacity to accumulate hemoglobin after shift to nonpermissive temperature. Our results support a model in which the transforming proteins of AEV, MC29, and MAV block the differentiation of their target cells by competitively inhibiting the action of a hypothetical homologous cellular differentiation protein synthesized in the corresponding target cells only.

MeSH Terms

Alpharetrovirus/genetics Animals Avian Leukosis Virus/genetics,pathogenicity Cell Differentiation Cell Transformation, Viral Chick Embryo Defective Viruses/genetics,pathogenicity Erythroblasts/microbiology Erythrocytes/microbiology Macrophages/microbiology Molecular Weight Viral Proteins/genetics Virus Replication

Chemicals

Viral Proteins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Graf T

Beug H

Hayman M J

References (21)

21 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.77.1.389

Published

1980-01-00

Pages

389-93

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC348276

Subset

External Links

PubMed source DOI: 10.1073/pnas.77.1.389 PMC Full Text View journal details

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Target cell specificity of defective avian leukemia viruses: hematopoietic target cells for a given virus type can be infected but not transformed by strains of a different type.

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