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PMID: 6234879 Published · ppublish English Journal Article

Primary structure of the calcium ion-transporting adenosine triphosphatase of rabbit skeletal sarcoplasmic reticulum. Soluble peptides from the alpha-chymotryptic digest of the carboxymethylated protein.

The Biochemical journal ·Vol. 187 ·No. 3 ·1980-06-01 ·Pages 565-75

Allen G

Abstract

The isolation of the soluble peptides from the chymotryptic digest of the calcium-transporting ATPase of rabbit skeletal sarcoplasmic reticulum is described. These peptides were partially sequenced and the information obtained was used to align tryptic peptides of the protein and to confirm sequences within the tryptic peptides. Details of the isolation of some peptides and the amino acid analyses of the peptides are given in Supplementary Publication SUP 50103 (10 pages), which has been deposited with the British Library Lending Division, Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1978) 169, 5.

MeSH Terms
Amino Acid Sequence Animals Calcium-Transporting ATPases/isolation & purification Carbon Radioisotopes Chymotrypsin Iodoacetates Iodoacetic Acid Muscles/enzymology Peptide Fragments/analysis Rabbits Sarcoplasmic Reticulum/enzymology
Chemicals
Carbon Radioisotopes Iodoacetates Peptide Fragments Chymotrypsin Calcium-Transporting ATPases Iodoacetic Acid
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Allen G
References (8)
8 references, click to expand
  1. Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups.
    Nature. 1966 Aug 6;211(5049):591-3 PMID: 5968723
  2. Studies on the location and orientation of proteins in the sarcoplasmic reticulum.
    Eur J Biochem. 1973 Dec 17;40(2):403-13 PMID: 4131254
  3. A 31-residue tryptic peptide from the active site of the [Ca++]-transporting adenosine triphosphatase of rabbit sarcoplasmic reticulum.
    FEBS Lett. 1976 Mar 15;63(1):188-92 PMID: 131039
  4. Primary structures of cysteine-containing peptides from the calcium ion-transporting adenosine triphosphatase of rabbit sarcoplasmic reticulum.
    Biochem J. 1978 Aug 1;173(2):393-402 PMID: 151533
  5. The primary structure of the calcium-transporting adenosine triphosphatase of rabbit skeletal sarcoplasmic reticulum. Soluble tryptic peptides from the succinylated carboxymethylated protein.
    Biochem J. 1980 Jun 1;187(3):545-63 PMID: 6234878
  6. The primary structure of the calcium ion-transporting adenosine triphosphatase protein of rabbit skeletal sarcoplasmic reticulum. Peptides derived from digestion with cyanogen bromide, and the sequences of three long extramembranous segments.
    Biochem J. 1980 Jun 1;187(3):591-616 PMID: 6234881
  7. A specific chemical difference between the globins of normal human and sickle-cell anaemia haemoglobin.
    Nature. 1956 Oct 13;178(4537):792-4 PMID: 13369537
  8. Peptide separation by two-dimensional chromatography and electrophoresis.
    J Biol Chem. 1959 Nov;234:2897-900 PMID: 14404782
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
Published
1980-06-01
Pages
565-75
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1162441
Subset
IM
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