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PMID: 6214561 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Centrifugation shearing exposes filamentous networks in cortical regions of crane-fly spermatocytes.

The Journal of cell biology ·Vol. 93 ·No. 3 ·1982-06-00 ·Pages 670-9

Strauch AR, LaFountain JR

Abstract

Spermatocytes of the crane-fly, Nephrotoma suturalis, were attached to electron microscope grids and then sheared by applying centrifugal force. Transmission electron microscopy of exposed regions of the cell cortex revealed networks containing arrays of filamentous structures. Networks were present in sheared spermatocytes at all stages of meiosis. The networks of dividing spermatocytes (meta- through telophase) were denser and appeared to contain more aggregated material then networks of prophase cells. The appearance of networks in spermatocytes resembled actin-containing networks of sheared and detergent-extracted human erythrocytes. Networks treated with myosin subfragment 1 under conditions in which muscle F-actin was clearly decorated were not distinguishable from those of untreated cells. Exposure to deoxyribonuclease-1 caused the disruption of networks in sheared spermatocytes as well as in erythrocytes. The results of deoxyribonuclease experiments are interpreted as an indication that actin is a component of the cell cortex in crane-fly spermatocytes.

MeSH Terms
Actins/metabolism Animals Cells, Cultured Centrifugation Cytoskeleton/drug effects,metabolism,ultrastructure Deoxyribonuclease I Diptera/metabolism Endodeoxyribonucleases/pharmacology Erythrocytes/metabolism,ultrastructure Humans Male Spermatocytes/metabolism,ultrastructure Spermatozoa/metabolism
Chemicals
Actins Endodeoxyribonucleases Deoxyribonuclease I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Strauch A R
LaFountain J R
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38 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.93.3.670
Published
1982-06-00
Pages
670-9
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2112173
Subset
IM
Grants
NIGMS NIH HHS · GM 27288 · United States
External Links
PubMed source DOI: 10.1083/jcb.93.3.670 PMC Full Text View journal details
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