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PMID: 6208382 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cleavage of nascent reovirus mRNA by localized activation of the 2'-5'-oligoadenylate-dependent endoribonuclease.

Journal of virology ·Vol. 52 ·No. 3 ·1984-12-00 ·Pages 865-71

Baglioni C, De Benedetti A, Williams GJ

Abstract

Addition of cell extracts prepared from untreated or interferon-treated cells reduced the yield of reovirus RNA synthesized in transcription reactions with purified virions. The reduction observed with the extract of interferon-treated cells was greater than that observed with the extract of untreated cells. The yield of reovirus RNA could be restored to the level obtained without addition of cell extracts by carrying out transcription either in the presence of an inhibitor of 2'-5'-oligoadenylate (2-5A) synthesis, 2'-dATP, or in the presence of the 3'-methyl analog of 5'-monophosphate 2-5A, which inhibits the 2-5A-dependent endoribonuclease. This enzyme was activated in a localized way since neither mRNA added to transcription reactions containing the extract of interferon-treated cells nor rRNA was appreciably degraded, whereas accumulation of reovirus mRNA was drastically inhibited. These findings provide evidence for an antiviral role of the 2-5A synthetase/endoribonuclease system, which preferentially cleaves nascent viral RNA.

MeSH Terms
2',5'-Oligoadenylate Synthetase/metabolism Endoribonucleases/metabolism Enzyme Activation Hydrolysis Interferons/pharmacology RNA, Messenger/metabolism RNA, Viral/metabolism Reoviridae/growth & development,metabolism
Chemicals
RNA, Messenger RNA, Viral Interferons 2',5'-Oligoadenylate Synthetase Endoribonucleases 2-5A-dependent ribonuclease
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Baglioni C
De Benedetti A
Williams G J
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26 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.52.3.865-871.1984
Published
1984-12-00
Pages
865-71
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254607
Subset
IM
Grants
NIAID NIH HHS · AI-16076 · United States
NIAID NIH HHS · AI-20429 · United States
External Links
PubMed source DOI: 10.1128/JVI.52.3.865-871.1984 PMC Full Text View journal details
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