Home LiteratureArticle Details
PMID: 6208205 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Expression of unusually large keratins during terminal differentiation: balance of type I and type II keratins is not disrupted.

The Journal of cell biology ·Vol. 99 ·No. 5 ·1984-11-00 ·Pages 1872-7

Kim KH, Marchuk D, Fuchs E

Abstract

When a basal epidermal cell undergoes a commitment to terminally differentiate, it ceases to divide and begins to migrate outward towards the surface of the skin. Dramatic changes in its cytoskeletal architecture take place, accompanied by numerous changes in the expression of keratins, a family of related polypeptides that form 8-nm filaments in these cells. We show here that a shift to the synthesis of unusually large keratins occurs that does not seem to disrupt the ratio of two distinct subfamilies of keratins. Preliminary studies indicate that this differentiation-specific shift may be at the level of transcriptional rather than post-transcriptional regulation. The striking similarities between these large keratins and the type I and type II keratins of basal epidermal cells suggests the important role that both classes of large keratin sequences must play in the assembly of the intermediate filaments within the differentiating keratinocyte.

MeSH Terms
Cell Differentiation Cells, Cultured DNA Epidermal Cells Epidermis/metabolism Humans Isoelectric Point Keratins/genetics,metabolism Molecular Weight Nucleic Acid Hybridization Poly A/metabolism RNA, Messenger/metabolism
Chemicals
RNA, Messenger Poly A Keratins DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kim K H
Marchuk D
Fuchs E
References (40)
40 references, click to expand
  1. Assembly of stratum corneum basic protein and keratin filaments in macrofibrils.
    Nature. 1978 Dec 14;276(5689):729-31 PMID: 732879
  2. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  3. Isolation of epidermal desmosomes.
    J Cell Biol. 1974 Nov;63(2 Pt 1):515-23 PMID: 4138144
  4. Chemical characterization of isolated epidermal desmosomes.
    J Cell Biol. 1974 Nov;63(2 Pt 1):524-30 PMID: 4421013
  5. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  6. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins of newbonr rat skin. II. Keratohyalin and stratum corneum proteins.
    J Invest Dermatol. 1975 Aug;65(2):223-7 PMID: 50389
  7. The identification of fibrous proteins in fetal rat epidermis by electrophoretic and immunologic techniques.
    J Invest Dermatol. 1976 Apr;66(4):230-5 PMID: 818319
  8. Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes.
    Nature. 1977 Feb 3;265(5593):421-4 PMID: 299924
  9. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  10. Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.
    Nucleic Acids Res. 1977;4(5):1539-52 PMID: 19730
  11. Fibrous protein of human epidermis.
    J Invest Dermatol. 1978 Aug;71(2):148-51 PMID: 681784
  12. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells.
    Cell. 1975 Nov;6(3):331-43 PMID: 1052771
  13. Structure and biochemical composition of desmosomes and tonofilaments isolated from calf muzzle epidermis.
    J Cell Biol. 1978 Nov;79(2 Pt 1):427-43 PMID: 569157
  14. Protein modifications during the keratinization of normal and psoriatic human epidermis.
    Biochim Biophys Acta. 1978 Dec 20;537(2):474-84 PMID: 728455
  15. The expression of keratin genes in epidermis and cultured epidermal cells.
    Cell. 1978 Nov;15(3):887-97 PMID: 728993
  16. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  17. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  18. Changes in keratin gene expression during terminal differentiation of the keratinocyte.
    Cell. 1980 Apr;19(4):1033-42 PMID: 6155214
  19. Experimental production of antibodies against stratum corneum keratin polypeptides.
    Arch Dermatol Res. 1980;267(2):179-88 PMID: 6157367
  20. Isolation and characterization of desmosome-associated tonofilaments from rat intestinal brush border.
    J Cell Biol. 1981 Jul;90(1):116-27 PMID: 6166624
  21. Isolation of the intercellular glycoproteins of desmosomes.
    J Cell Biol. 1981 Jul;90(1):243-8 PMID: 6166625
  22. Regulation of terminal differentiation of cultured human keratinocytes by vitamin A.
    Cell. 1981 Sep;25(3):617-25 PMID: 6169442
  23. Two distinct classes of keratin genes and their evolutionary significance.
    Cell. 1981 Nov;27(1 Pt 2):75-84 PMID: 6173133
  24. Modification of human prekeratin during epidermal differentiation.
    Biochem J. 1981 Oct 1;199(1):145-54 PMID: 6175314
  25. Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies.
    J Cell Biol. 1982 Nov;95(2 Pt 1):580-8 PMID: 6183275
  26. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells.
    Cell. 1982 Nov;31(1):11-24 PMID: 6186379
  27. The cDNA sequence of a human epidermal keratin: divergence of sequence but conservation of structure among intermediate filament proteins.
    Cell. 1982 Nov;31(1):243-52 PMID: 6186381
  28. Tonofilament differentiation in human epidermis, isolation and polypeptide chain composition of keratinocyte subpopulations.
    Exp Cell Res. 1983 Jan;143(1):27-35 PMID: 6186509
  29. Keratin gene expression in mouse epidermis and cultured epidermal cells.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):716-20 PMID: 6187003
  30. Structural homology between hard alpha-keratin and the intermediate filament proteins desmin and vimentin.
    Biosci Rep. 1983 Jan;3(1):73-8 PMID: 6188504
  31. Complete amino acid sequence of a mouse epidermal keratin subunit and implications for the structure of intermediate filaments.
    Nature. 1983 Apr 28;302(5911):794-800 PMID: 6188955
  32. Characterization of the chicken vimentin gene: single copy gene producing multiple mRNAs.
    Proc Natl Acad Sci U S A. 1983 Feb;80(4):911-5 PMID: 6573660
  33. Tissue specificity of epithelial keratins: differential expression of mRNAs from two multigene families.
    Mol Cell Biol. 1983 Apr;3(4):495-502 PMID: 6190074
  34. The cDNA sequence of a Type II cytoskeletal keratin reveals constant and variable structural domains among keratins.
    Cell. 1983 Jul;33(3):915-24 PMID: 6191871
  35. Type I and type II keratins have evolved from lower eukaryotes to form the epidermal intermediate filaments in mammalian skin.
    Proc Natl Acad Sci U S A. 1983 Oct;80(19):5857-61 PMID: 6193525
  36. Unraveling the structure of the intermediate filaments.
    Cell. 1983 Sep;34(2):332-4 PMID: 6352049
  37. Tissue-specific expression of two mRNA species transcribed from a single vimentin gene.
    Cell. 1983 Dec;35(2 Pt 1):411-20 PMID: 6317186
  38. Significance of two desmosome plaque-associated polypeptides of molecular weights 75 000 and 83 000.
    EMBO J. 1983;2(12):2211-5 PMID: 6199193
  39. Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression.
    J Cell Biol. 1984 Apr;98(4):1388-96 PMID: 6201491
  40. Sequence of a cDNA clone encoding mouse glial fibrillary acidic protein: structural conservation of intermediate filaments.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2743-6 PMID: 6585825
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.99.5.1872
Published
1984-11-00
Pages
1872-7
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113356
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.99.5.1872 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2