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PMID: 6208132 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of monoclonal antibody specific for human alpha interferon.

Hybridoma ·Vol. 1 ·No. 3 ·1982-00-00 ·Pages 313-22

Laurent AG, Gruest J, Krust B, Montagnier L

Abstract

A mouse hybridoma cell line has been isolated, which secretes a monoclonal antibody specific of human leukocyte (alpha) interferon. The antibody secreted by the hybridoma belongs to the IgG1 class. It neutralizes biological activities (cellular and antiviral) of alpha interferon. Mass production of the antibody in ascitic fluid has been obtained. A convenient method of purification of the IgG from the ascitic fluid on DEAE-Trisacryl M is described.

MeSH Terms

Animals Antibodies, Monoclonal/biosynthesis,immunology,isolation & purification Antibody Affinity Antibody Specificity Cross Reactions Epitopes Humans Hybridomas Immunoglobulin G/immunology,isolation & purification Interferon Type I/immunology Mice Molecular Weight Neutralization Tests

Chemicals

Antibodies, Monoclonal Epitopes Immunoglobulin G Interferon Type I

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Laurent A G

Gruest J

Krust B

Montagnier L

Article Info

Journal

Hybridoma

Abbr.

Hybridoma

ISSN

0272-457X

DOI

10.1089/hyb.1.1982.1.313

Published

1982-00-00

Pages

313-22

Language

English

Region

United States

NLM ID

8202424

Subset

External Links

PubMed source DOI: 10.1089/hyb.1.1982.1.313

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