Home LiteratureArticle Details
PMID: 6200440 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Monoclonal antibodies reactive with all strains of Haemophilus ducreyi.

Infection and immunity ·Vol. 44 ·No. 1 ·1984-04-00 ·Pages 196-8

Hansen EJ, Loftus TA

Abstract

We have isolated plasma cell hybridomas which secrete monoclonal antibodies directed against Haemophilus ducreyi. Two of these monoclonal antibodies recognize all strains of H. ducreyi tested to date and are capable of detecting the presence of H. ducreyi in skin lesions produced by this pathogen in experimental animals. These monoclonal antibodies which react with apparently all strains of H. ducreyi have the potential to be developed into a rapid immunodiagnostic test for chancroid.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antibody Specificity Antigens, Bacterial/immunology Chancroid/diagnosis Epitopes/immunology Haemophilus ducreyi/immunology Hybridomas/immunology Immunoassay Mice Mice, Inbred BALB C Rabbits Serologic Tests Species Specificity
Chemicals
Antibodies, Monoclonal Antigens, Bacterial Epitopes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hansen E J
Loftus T A
References (22)
22 references, click to expand
  1. Chancroid: alias the soft chancre.
    Int J Dermatol. 1975 Apr;14(3):188-97 PMID: 1092626
  2. Comparison of specimen collection and laboratory techniques for isolation of Haemophilus ducreyi.
    J Clin Microbiol. 1978 Jan;7(1):39-43 PMID: 342538
  3. Antimicrobial susceptibility of Haemophilus ducreyi.
    Antimicrob Agents Chemother. 1978 Apr;13(4):608-12 PMID: 307364
  4. An indirect fluorescent antibody technique for Haemophilus ducreyi.
    Health Lab Sci. 1978 Jul;15(3):128-32 PMID: 359505
  5. Radioimmunological screening method for specific membrane proteins.
    Anal Biochem. 1979 Aug;97(1):153-7 PMID: 384844
  6. Rising incidence of chancroid in Rotterdam. Epidemiological, clinical, diagnostic, and therapeutic aspects.
    Br J Vener Dis. 1979 Dec;55(6):439-41 PMID: 526847
  7. Isolation and identification of Haemophilus ducreyi in a clinical study.
    J Clin Microbiol. 1980 Aug;12(2):170-4 PMID: 6971872
  8. Serological analysis of herpes simplex virus types 1 and 2 with monoclonal antibodies.
    Infect Immun. 1982 Jan;35(1):363-7 PMID: 6172383
  9. Genital ulcers in Kenya. Clinical and laboratory study.
    Br J Vener Dis. 1981 Dec;57(6):378-81 PMID: 6799142
  10. Chancroid in Sheffield. A report of 22 cases diagnosed by isolating Haemophilus ducreyi in a modified medium.
    Br J Vener Dis. 1981 Dec;57(6):382-6 PMID: 6976815
  11. Monoclonal antibodies to Chlamydia trachomatis: antibody specificities and antigen characterization.
    J Immunol. 1982 Mar;128(3):1083-9 PMID: 7035557
  12. Modified haemin-containing medium for isolation of Haemophilus ducreyi.
    Lancet. 1982 Feb 13;1(8268):393-4 PMID: 6120368
  13. Isolation and cultivation of Haemophilus ducreyi.
    J Clin Microbiol. 1982 Apr;15(4):625-9 PMID: 6802870
  14. Monoclonal antibodies directed against a cell surface-exposed outer membrane protein of Haemophilus influenzae type b.
    Infect Immun. 1982 Apr;36(1):80-8 PMID: 6176548
  15. Murine monoclonal antibodies specific for virulent Treponema pallidum (Nichols).
    Infect Immun. 1982 Jun;36(3):1076-85 PMID: 7047388
  16. Characteristics of Haemophilus ducreyi. A study.
    Br J Vener Dis. 1982 Aug;58(4):239-42 PMID: 6980682
  17. Antibody response of infants to cell surface-exposed outer membrane proteins of Haemophilus influenzae type b after systemic Haemophilus disease.
    Infect Immun. 1982 Jul;37(1):82-8 PMID: 6980838
  18. Treatment of chancroid by clavulanic acid with amoxycillin in patients with beta-lactamase-positive Haemophilus ducreyi infection.
    Lancet. 1982 Sep 4;2(8297):509-11 PMID: 6125676
  19. Starch aggregation as a presumptive test for Haemophilus ducreyi.
    Lancet. 1982 Oct 16;2(8303):872 PMID: 6181362
  20. Enzyme immunoassays for the detection of bacterial antigens utilizing biotin-labeled antibody and peroxidase biotin--avidin complex.
    J Immunol Methods. 1983 Feb 11;56(3):319-27 PMID: 6339630
  21. A set of two monoclonal antibodies specific for the cell surface-exposed 39K major outer membrane protein of Haemophilus influenzae type b defines all strains of this pathogen.
    Infect Immun. 1983 Nov;42(2):516-24 PMID: 6605924
  22. Isolation of Haemophilus species from the genital tract.
    Sex Transm Dis. 1983 Apr-Jun;10(2):56-61 PMID: 6658630
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.44.1.196-198.1984
Published
1984-04-00
Pages
196-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC263493
Subset
IM
Grants
NIAID NIH HHS · AI-17012 · United States
NIAID NIH HHS · AI-17621 · United States
External Links
PubMed source DOI: 10.1128/iai.44.1.196-198.1984 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2