Simultaneous localization of two different tissue antigens based on the silver intensified PAP-DAB and on the traditional PAP-DAB method.

A new immunohistological method was elaborated for the light-microscopic detection of two tissue antigens in the same section. One of the antigens was visualized by the silver-gold intensified peroxidase-antiperoxidase complex (PAP)-diaminobenzidine (DAB) method resulting in a black coloured end-product. The other antigen was labelled by the brown reaction-product of the traditional PAP-DAB method. Both reaction-products are stable, electron-dense and they can clearly be distinguished from each other.