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PMID: 6192042 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Isolation and characterization of three cloned fragments of human DNA coding for tRNAs and small nuclear RNA U1.

Gene ·Vol. 22 ·No. 2-3 ·1983-00-00 ·Pages 211-7

Buckland RA, Cooke HJ, Roy KL, Dahlberg JE, Lund E

Abstract

Employing a human fetal liver library in lambda Charon 4A phage vector, we have isolated and characterized three clones of human DNA containing genes for tRNAs. One clone contains at least three tRNA genes (tRNALys, tRNAGln and tRNALeu) within 2 kb of each other. The other two clones contain two different single genes for tRNAAsn. One of these latter two DNAs also contains a gene for U1 small nuclear RNA.

MeSH Terms

Base Sequence Cloning, Molecular DNA/isolation & purification DNA Restriction Enzymes Female Fetus Humans Liver/embryology,metabolism Pregnancy RNA/genetics RNA, Small Nuclear RNA, Transfer/genetics

Chemicals

RNA, Small Nuclear RNA DNA RNA, Transfer DNA Restriction Enzymes

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Buckland R A

Cooke H J

Roy K L

Dahlberg J E

Lund E

Article Info

Journal

Gene

Abbr.

Gene

ISSN

0378-1119

DOI

10.1016/0378-1119(83)90105-1

Published

1983-00-00

Pages

211-7

Language

English

Region

Netherlands

NLM ID

7706761

Subset

External Links

PubMed source DOI: 10.1016/0378-1119(83)90105-1 View journal details

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Isolation and characterization of three cloned fragments of human DNA coding for tRNAs and small nuclear RNA U1.

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