A consistent picture of the actin filament related to the orientation of the actin molecule.
We show that freeze-dried actin filaments which have been rotary shadowed with a light coat of platinum appear very similar in morphology and width to negatively-stained filaments. The addition of a thicker coat of platinum to such preparations gives the actin filaments a different morphology and width, which are similar to those of the rotary-shadowed, quick-frozen filaments described by Heuser and Kirschner (J. Cell Biol. 1980, 86:212-234). The consistent view of the actin filament presented here, particularly its 7-8-nm width, can be interpreted in terms of the overall orientation of the actin subunit in the actin filament.
18 references, click to expand
-
The lattice spacing of crystalline catalase as an internal standard of length in electron microscopy.J Ultrastruct Res. 1968 Sep;24(5):454-64 PMID: 5751523
-
Three-dimensional reconstruction of F-actin, thin filaments and decorated thin filaments.J Mol Biol. 1970 Jun 14;50(2):279-95 PMID: 5476917
-
Regulation of skeletal muscle contraction. II. Structural studies of the interaction of the tropomyosin-troponin complex with actin.J Mol Biol. 1972 Dec 30;72(3):619-32 PMID: 4349760
-
Electron microscopy of the stacked disk aggregate of tobacco mosaic virus protein. II. The influence of electron irradiation of the stain distribution.J Mol Biol. 1974 Aug 25;87(4):657-70 PMID: 4139268
-
Three-dimensional image reconstruction of actin-tropomyosin complex and actin-tropomyosin-troponin T-troponin I complex.J Mol Biol. 1975 Apr 25;93(4):477-97 PMID: 1142432
-
Freeze drying and shadowing a two-dimensional periodic specimen.J Ultrastruct Res. 1977 Apr;59(1):76-86 PMID: 66324
-
Structure of actin-containing filaments from two types of non-muscle cells.J Mol Biol. 1977 Jul 15;113(4):679-95 PMID: 561192
-
Apparent holes in rotary shadowed proteins: dependence on angle of shadowing and replica thickness.J Microsc. 1979 Nov;117(2):313-5 PMID: 399302
-
Filament organization revealed in platinum replicas of freeze-dried cytoskeletons.J Cell Biol. 1980 Jul;86(1):212-34 PMID: 6893451
-
Freeze-drying specimens for electron microscopy.J Ultrastruct Res. 1980 Sep;72(3):380-4 PMID: 6107386
-
Structure of F-actin needles from extracts of sea urchin oocytes.J Mol Biol. 1981 Feb 15;146(1):77-99 PMID: 6894946
-
Three-dimensional structure of the complex of skeletal muscle actin and bovine pancreatic DNAse I at 6-A resolution.Proc Natl Acad Sci U S A. 1981 Jul;78(7):4319-23 PMID: 6270671
-
Crystalline actin sheets: their structure and polymorphism.J Cell Biol. 1981 Nov;91(2 Pt 1):340-51 PMID: 7309785
-
F-actin is a helix with a random variable twist.Nature. 1982 Jul 8;298(5870):131-5 PMID: 7201078
-
Polymorphism of actin paracrystals induced by polylysine.J Cell Biol. 1982 May;93(2):452-8 PMID: 7096448
-
Role of fimbrin and villin in determining the interfilament distances of actin bundles.Nature. 1983 Jan 20;301(5897):209-14 PMID: 6823301
-
Crystallographic studies of the chicken gizzard G-actin X DNase I complex at 5A resolution.J Biochem. 1983 Jan;93(1):299-302 PMID: 6221013
-
F-ACTIN IS A RIGHT-HANDED HELIX.J Mol Biol. 1965 May;12:302-3 PMID: 14343294
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
