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PMID: 6182146 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Continuous growth of proximal tubular kidney epithelial cells in hormone-supplemented serum-free medium.

The Journal of cell biology ·Vol. 94 ·No. 3 ·1982-09-00 ·Pages 506-10

Chuman L, Fine LG, Cohen AH, Saier MH

Abstract

An epithelial cell line from pig kidney (LLC-PK1) with properties of proximal tubular cells can be maintained indefinitely in hormone-supplemented serum-free medium. Continuous growth requires the presence of seven factors: transferrin, insulin, selenium, hydrocortisone, triiodothyronine, vasopressin, and cholesterol. The hormone-defined medium (a) supports growth of LLC-PK1 cells at a rate of approaching that observed in serum-supplemented medium; (b) allows vectorial transepithelial salt and fluid transport as measured by hemicyst formation; and (c) influences cell morphology. The vasopressin dependency for growth and morphology can be partially replaced by isobutylmethylxanthine or dibutyryl cyclic AMP. The medium has been used to isolate rabbit proximal tubular kidney epithelial cells free of fibroblasts.

MeSH Terms
1-Methyl-3-isobutylxanthine/pharmacology Animals Bucladesine/pharmacology Cell Division/drug effects Cells, Cultured Culture Media Epithelium Hormones/pharmacology Intercellular Junctions/ultrastructure Kidney Tubules, Proximal/cytology Rabbits
Chemicals
Culture Media Hormones Bucladesine 1-Methyl-3-isobutylxanthine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chuman L
Fine L G
Cohen A H
Saier M H
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21 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.94.3.506
Published
1982-09-00
Pages
506-10
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2112226
Subset
IM
Grants
NIADDK NIH HHS · AM21994-03 · United States
External Links
PubMed source DOI: 10.1083/jcb.94.3.506 PMC Full Text View journal details
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