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PMID: 6181502 Published · ppublish English Journal Article

Expression of a chemically synthesized human alpha 1 interferon gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 14 ·1982-07-00 ·Pages 4256-9

De Maeyer E, Skup D, Prasad KS, De Maeyer-Guignard J, Williams B, Meacock P, Sharpe G, Pioli D, Hennam J, Schuch W, Atherton K

Abstract

Cells of Escherichia coli containing a chemically synthesized human alpha 1 interferon (IFN-alpha 1) gene, under control of the lac promoter, make a product with biological properties indistinguishable from those of the natural IFN-alpha 1 [antiviral activity, acid stability, species crossreactivity, inactivation by antisera directed against leukocyte or Namalwa cell interferon, and stimulation of (2'-5')oligoadenylate synthetase activity]. Similar levels of IFN synthesis were obtained when the expression unit (lac promoter plus synthetic IFN-alpha 1 gene) was transplanted into the obligate methylotroph Methylophilus methylotrophus.

MeSH Terms
2',5'-Oligoadenylate Synthetase/metabolism Biological Assay DNA, Recombinant Enzyme Activation/drug effects Escherichia coli/genetics Gene Expression Regulation Genes, Synthetic Humans Hydrogen-Ion Concentration Interferons/genetics,pharmacology Methylococcaceae/genetics Plasmids
Chemicals
DNA, Recombinant Interferons 2',5'-Oligoadenylate Synthetase
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
De Maeyer E
Skup D
Prasad K S
De Maeyer-Guignard J
Williams B
Meacock P
Sharpe G
Pioli D
Hennam J
Schuch W
Atherton K
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15 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.14.4256
Published
1982-07-00
Pages
4256-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346649
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.14.4256 PMC Full Text View journal details
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