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PMID: 6180976 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Monoclonal antibodies (McAbs) to determinants on human gamma chains: properties of antibodies showing subclass restriction or subclass specificity.

Immunology ·Vol. 47 ·No. 2 ·1982-10-00 ·Pages 329-36

Lowe J, Bird P, Hardie D, Jefferis R, Ling NR

Abstract

Thirteen monoclonal antibodies (McAbs) showing subclass-specific and subclass-restricted reactivity to human IgG have been produced by the hybridoma technique. Their specificity has been defined by passive haemagglutination (HA) and haemagglutination-inhibition (HAI) assays using a large panel pf purified IgG paraproteins of known subclass. Their use in assaying human IgG subclasses in radial immunodiffusion (RID) and in purifying human subclasses by both positive and negative immunoabsorption chromatography is illustrated.

MeSH Terms

Animals Antibodies, Monoclonal/classification,immunology Antibody Specificity Chromatography, Affinity Epitopes/immunology Hemagglutination Tests Humans Immunodiffusion Immunoglobulin G/classification,immunology Mice Mice, Inbred BALB C

Chemicals

Antibodies, Monoclonal Epitopes Immunoglobulin G

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Lowe J

Bird P

Hardie D

Jefferis R

Ling N R

References (15)

15 references, click to expand

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Article Info

Journal

Immunology

Abbr.

Immunology

ISSN

0019-2805

Published

1982-10-00

Pages

329-36

Language

English

Region

England

NLM ID

0374672

PMCID

PMC1555448

Subset

External Links

PubMed source PMC Full Text View journal details

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Monoclonal antibodies (McAbs) to determinants on human gamma chains: properties of antibodies showing subclass restriction or subclass specificity.

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