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PMID: 6179096 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of a cDNA sequence encoding a trophectoderm-specific marker during mouse blastocyst formation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 7 ·1982-04-00 ·Pages 2328-32

Brûlet P, Jacob F

Abstract

A cDNA clone has been isolated from a trophoblastoma cDNA library. The mRNA complementary to this sequence directs the in vitro synthesis of proteins. The two-dimensional electrophoretic pattern of migration of these proteins is superposable to that of the trophoblastoma intermediate filament proteins recognized by monoclonal antibody (mAb) TROMA-1. This mAb had previously been shown to label trophectoderm cells but not inner cell mass cells. With a sensitive binding assay (ultrasensitive enzymatic radioimmunoassay), these in vitro synthesized proteins were recognized by mAb TROMA-1. The proteins are immunoprecipitated by an antiserum directed against trophoblastoma intermediate filament proteins and by a serum directed against a major cytoskeletal protein found in murine extraembryonic endodermal cell lines (Endo-A) [Oshima R. G. (1981) J. Biol. Chem. 256, 8124-8133]. The cDNA sequence detects specific mRNA(s) migrating with 18S ribosomal RNA in trophoblastoma but not in embryonal carcinoma cells.

MeSH Terms
Animals Antibodies, Monoclonal Blastocyst/metabolism Cell Line Cloning, Molecular DNA/genetics Electrophoresis, Polyacrylamide Gel Genetic Markers Protein Biosynthesis RNA/genetics RNA, Messenger/genetics Rabbits Teratoma
Chemicals
Antibodies, Monoclonal Genetic Markers RNA, Messenger RNA DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brûlet P
Jacob F
References (17)
17 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.7.2328
Published
1982-04-00
Pages
2328-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346186
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.7.2328 PMC Full Text View journal details
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