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PMID: 6178107 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Monoclonal antibodies that recognize discrete forms of tubulin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 8 ·1982-04-00 ·Pages 2579-83

Gozes I, Barnstable CJ

Abstract

Anti-tubulin antibodies secreted by plasmacytoma NSI-spleen cell hybrids were detected by an indirect binding assay. Different antibodies bound to different combinations of the tubulins as resolved by isoelectric focusing. Two monoclonal antibodies (TUB 2.1 and TUB 2.5) labeled only (i) the tubulin band on a polyacrylamide electropherogram and (ii) beta-tubulins as resolved by isoelectric focusing. The fraction that was specifically bound and eluted from antibody affinity columns was enriched in beta-tubulins as compared with alpha-tubulins, suggesting the possibility of some soluble tubulin homodimers and alpha,beta-heterodimers. Double labeling experiments were used to show that all detectable microtubules contained beta-tubulin.

MeSH Terms
Animals Antibodies, Monoclonal Antigen-Antibody Complex Cell Fusion Cell Line Chromatography, Affinity Epitopes/analysis Fluorescent Antibody Technique Male Mice Mice, Inbred BALB C Molecular Weight Neurons/cytology Plasmacytoma/immunology Rats Rats, Inbred Strains Tubulin/analysis,immunology
Chemicals
Antibodies, Monoclonal Antigen-Antibody Complex Epitopes Tubulin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gozes I
Barnstable C J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.8.2579
Published
1982-04-00
Pages
2579-83
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346243
Subset
IM
Grants
NEI NIH HHS · EY01995 · United States
NINDS NIH HHS · NS 17309 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.8.2579 PMC Full Text View journal details
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