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PMID: 6174669 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Specificity studies on cytolytic T lymphocytes directed against murine leukemia virus-induced tumors. Analysis of monoclonal cytolytic T lymphocytes.

The Journal of experimental medicine ·Vol. 155 ·No. 4 ·1982-04-01 ·Pages 1050-62

Plata F

Abstract

The specificities of cloned cytolytic T lymphocytes (CTL) were studied for the analysis of CTL populations generated against murine leukemia viruses (MuLV) in H-2 congenic BALB/c (H-2d) and BALB.B (H-2b) mice. In particular, CTL generated in response to tumors induced by Gross MuLV and Friend MuLV were studied; these tumors expressed virus-induced antigens that do not cross-react and that can be distinguished from each other. The systematic study of 92 CTL clones clearly indicated that MuLV-immune CTL were highly heterogeneous with respect to both the intensities of target cell lysis that they mediated and to their specificity of recognition of MuLV-induced tumor target cells. Various categories of CTL clones were identified, ranging from CTL clones tht were tightly H-2 restricted and specific for the immunizing tumor to CTL clones that displayed no discernible patterns of specificity and that attacked a large number of different target cells. In addition, the surface markers of these cloned CTL were defined, and the best conditions for their prolonged maintenance in culture were determined. The present data indicate that future efforts in the definition of target antigens recognized by tumor-specific CTL should be performed with monoclonal lymphocytes.

MeSH Terms
Animals Antigens, Ly Antigens, Surface Clone Cells/immunology Cross Reactions Cytotoxicity, Immunologic Epitopes Leukemia Virus, Murine/immunology Leukemia, Experimental/immunology Lymphocyte Culture Test, Mixed Membrane Proteins Mice Mice, Inbred BALB C Mice, Inbred C57BL Receptors, Antigen, B-Cell T-Lymphocytes/immunology Thy-1 Antigens
Chemicals
Antigens, Ly Antigens, Surface Epitopes Membrane Proteins Receptors, Antigen, B-Cell Thy-1 Antigens
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Plata F
References (37)
37 references, click to expand
  1. The G (Gross) leukemia antigen.
    Cancer Res. 1965 Jul;25(6):813-9 PMID: 4284252
  2. Cross-resistance to the transplantation of syngeneic Friend, Moloney, and Rauscher virus-induced tumors.
    Cancer Res. 1968 Mar;28(3):434-9 PMID: 4867923
  3. Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs.
    Immunology. 1968 Feb;14(2):181-96 PMID: 4966657
  4. Specificity of 51Cr-release cytotoxicity of lymphocytes immune to murine sarcoma virus.
    J Natl Cancer Inst. 1974 Oct;53(4):1103-11 PMID: 4139276
  5. Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity.
    J Exp Med. 1975 Jun 1;141(6):1390-9 PMID: 1092799
  6. Properties of cell lines derived from tumors induced by Friend virus in BALB/c and BALB/c-H-2b mice.
    J Exp Med. 1975 Jul 1;142(1):212-23 PMID: 1056976
  7. T-lymphocytes response to Friend virus-induced tumour cell lines in mice of strains congenic at H--2.
    Nature. 1976 Mar 18;260(5548):250-2 PMID: 1082989
  8. Murine sarcoma virus (MSV)-induced tumors in mice. I. Distribution of MSV-immune cytolytic T lymphocytes in vivo.
    Int J Cancer. 1977 Feb 15;19(2):205-11 PMID: 300075
  9. Characterization to effector lymphocytes associated with immunity to murine sarcoma virus (MSV)-induced tumors. II. Repeated stimulation and proliferation in vitro of specific cytolytic T lymphocytes.
    J Immunol. 1977 Aug;119(2):623-9 PMID: 196025
  10. Exclusive involvement of H-2Db or H-2Kd product in the interaction between T-killer lymphocytes and syngeneic H-2b or H-2d viral lymphomas.
    J Exp Med. 1977 Oct 1;146(4):909-22 PMID: 197198
  11. Studies on the nature of the cell surface antigen reacting with cytolytic T lymphocytes in murine oncornavirus-induced tumors.
    Eur J Immunol. 1978 Apr;8(4):228-36 PMID: 78851
  12. Origin of spleen colonies generated by Friend virus-infected cells in mice.
    Cancer Res. 1978 Sep;38(9):2729-33 PMID: 679178
  13. Translational products of type-C RNA tumor viruses.
    Adv Cancer Res. 1978;27:1-53 PMID: 80099
  14. Antigenic specificities of the cellular immune response of C57BL/6 mice to the Moloney leukemia/sarcoma virus complex.
    J Immunol. 1979 Feb;122(2):665-74 PMID: 84033
  15. Monoclonal cytolytic T-cell lines.
    J Exp Med. 1979 Jan 1;149(1):273-8 PMID: 310861
  16. Cell-mediated immunity to antigens associated with murine sarcoma virus-induced tumors: augmentation of cytolytic T lymphocyte activity by successive specific and nonspecific stimulation in vitro.
    Eur J Immunol. 1979 Mar;9(3):179-84 PMID: 221224
  17. Mechanisms of leukemogenesis. I. Generation of autoreactive lymphocytes in response to a murine leukemia virus.
    J Immunol. 1979 May;122(5):1874-80 PMID: 221580
  18. Fine specificity of a continuously growing killer cell clone specific for H-Y antigen.
    Eur J Immunol. 1979 Aug;9(8):592-7 PMID: 91527
  19. Viral specificity of H-2-restricted T killer cells directed against syngeneic tumors induced by Gross, Friend, or Rauscher leukemia virus.
    J Exp Med. 1979 Nov 1;150(5):1174-86 PMID: 91655
  20. T cell-mediated immunity to oncornavirus-induced tumors. II. Ability of different T cell sets to prevent tumor growth in vivo.
    J Immunol. 1980 Feb;124(2):851-4 PMID: 6243333
  21. Studies of cloned Friend erythroleukemia tumor cells. Modulation of the tumor-specific cytologic T lymphocyte response by infectious Friend virus production in vitro.
    J Exp Med. 1980 Mar 1;151(3):726-42 PMID: 6965703
  22. Antigenic specificity of the cytolytic T lymphocyte (CTL) response to murine sarcoma virus-induced tumors. II. Analysis of the clonal progeny of CTL precursors stimulated in vitro with syngeneic tumor cells.
    J Immunol. 1980 Apr;124(4):1627-34 PMID: 6154076
  23. Specificity of cytolytic T cells directed against AKR/Gross virus-induced syngeneic leukemias: antibodies directed against H-2K, but not against viral proteins, inhibit lysis.
    J Immunol. 1980 Aug;125(2):647-55 PMID: 6156211
  24. Cytotoxic T lymphocyte recognition of gp70 on Friend virus-induced erythroleukemia cell clones.
    J Immunol. 1980 Sep;125(3):1318-24 PMID: 6967898
  25. T-cell growth factor.
    Immunol Rev. 1980;51:337-57 PMID: 7000676
  26. H-2-restricted cytolytic T lymphocytes specific for a subclass of AKR endogenous leukemia virus-induced tumors: correlation of tumor cell susceptibility with expression of the gross cell surface antigen.
    J Immunol. 1980 Dec;125(6):2584-90 PMID: 6159413
  27. Antigenic specificity of the cytolytic T lymphocyte response to murine sarcoma virus-induced tumors. III. Characterization of cytolytic T lymphocyte clones specific for Moloney leukemia virus-associated cell surface antigens.
    J Exp Med. 1980 Nov 1;152(5):1210-25 PMID: 6159445
  28. Alloreactivity of an antigen-specific T-cell clone.
    Nature. 1980 Oct 30;287(5785):855-7 PMID: 6968869
  29. Cytotoxic T cell responses to haptenated cells. III. Isolation and specificity analysis of continuously growing clones.
    Eur J Immunol. 1980 Nov;10(11):828-34 PMID: 6970128
  30. Murine T lymphocyte clones with distinct immunological functions.
    Immunol Rev. 1981;54:225-66 PMID: 6166535
  31. Heterogeneity and specificity of cloned lines of influenza-virus specific cytotoxic T lymphocytes.
    J Exp Med. 1981 Apr 1;153(4):910-23 PMID: 6166708
  32. Simultaneous expression of H-2-restricted and alloreactive recognition by a cloned line of influenza virus-specific cytotoxic T lymphocytes.
    J Exp Med. 1981 May 1;153(5):1371-6 PMID: 6166717
  33. Cultured T lymphocytes cytotoxic for a syngeneic lymphoma: derivation in Con A-conditioned medium and in vivo activity.
    Clin Exp Immunol. 1981 Apr;44(1):137-44 PMID: 6973429
  34. Quantitation and clonal isolation of cytolytic T lymphocyte precursors selectively infiltrating murine sarcoma virus-induced tumors.
    J Exp Med. 1981 Aug 1;154(2):362-73 PMID: 6973602
  35. Quantitative variations in the expression of H-2 antigens on murine leukemia virus-induced tumor cells can affect the H-2 restriction patterns of tumor-specific cytolytic T lymphocytes.
    J Exp Med. 1981 Dec 1;154(6):1795-810 PMID: 6172533
  36. STUDIES ON INDUCED RESISTANCE AGAINST ISOTRANSPLANTS OF VIRUS-INDUCED LEUKEMIA.
    Cancer Res. 1964 Apr;24:502-8 PMID: 14147826
  37. TYPING OF MOUSE LEUKAEMIAS BY SEROLOGICAL METHODS.
    Nature. 1964 Feb 22;201:777-9 PMID: 14161198
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.155.4.1050
Published
1982-04-01
Pages
1050-62
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2186662
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.155.4.1050 PMC Full Text View journal details
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