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PMID: 6172447 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of the suppressor cell activity in human cord blood lymphocytes.

The Journal of clinical investigation ·Vol. 68 ·No. 6 ·1981-12-00 ·Pages 1577-85

Rodriguez MA, Bankhurst AD, Ceuppens JL, Williams RC

Abstract

Newborn mononuclear cells are known to have increased suppressor activity when compared with adult cells. However, the precise phenotypic description of the suppressor cell subpopulation has not yet been reported. This study was designed to examine the surface markers on human cord blood cells as defined by monoclonal antibodies and to characterize cord blood mononuclear cells that suppress in vitro pokeweed mitogen-driven immunoglobulin production. The pattern of fluorescence with the 9,6-monoclonal antibody suggested either a decreased density or a partial blocking of E-receptors on cord blood lymphocytes. Otherwise, human cord and adult cells had similar proportions of T cell subpopulations. Different subsets of newborn cells isolated by a monoclonal antibody rosetting technique were tested for suppressor activity. Cord blood lymphocytes recognized by the OKT8 monoclonal antibody constituted the major functional suppressor cell subpopulation. These cells were present in both E-rosetting and non-E-rosetting populations. Removal of OKT8+ mononuclear cord blood cells abrogated most of the suppressor activity. Thus the major suppressor cell present in cord blood is an OKT8 positive lymphocyte.

MeSH Terms
Adult Antibodies, Monoclonal/immunology Cell Membrane/immunology,ultrastructure Cell Separation Cells, Cultured Epitopes/analysis Fetal Blood/immunology Flow Cytometry Humans Immunoglobulins/biosynthesis Infant, Newborn Mitogens/pharmacology Phenotype Rosette Formation T-Lymphocytes, Regulatory/drug effects,immunology,ultrastructure
Chemicals
Antibodies, Monoclonal Epitopes Immunoglobulins Mitogens
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rodriguez M A
Bankhurst A D
Ceuppens J L
Williams R C
References (34)
34 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/jci110412
Published
1981-12-00
Pages
1577-85
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC370962
Subset
IM
Grants
NIADDK NIH HHS · AM 13690 · United States
NCI NIH HHS · CA 24873 · United States
External Links
PubMed source DOI: 10.1172/jci110412 PMC Full Text View journal details
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