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PMID: 6170981 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

cDNA sequence of a new chicken embryonic rho-globin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 8 ·1981-08-00 ·Pages 4782-5

Roninson IB, Ingram VM

Abstract

In order to use specific DNA probes for the study of developmentally regulated gene expression, we have prepared cDNA clones corresponding to chicken embryonic globins by inserting cDNA.mRNA hybrids into the Pst I site of the plasmid pBR322 by using poly(dG) and poly(dC) linkers. The nucleotide sequence of the insert of one clone, representing a nearly full-length copy of an embryonic beta-like globin cDNA, has been determined. The amino acid sequence of the globin encoded by this insert is identical to the sequence of embryonic rho-globin, except for four amino acid residues near the carboxy terminus. Comparison of mRNA sequences of the embryonic and adult chicken beta-globins indicates the presence of extensive deletions in the 3' untranslated region of the embryonic gene.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chick Embryo Chickens/genetics Cloning, Molecular/methods DNA/genetics Fetal Hemoglobin/genetics Globins/genetics Plasmids RNA, Messenger/genetics
Chemicals
RNA, Messenger Globins DNA Fetal Hemoglobin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Roninson I B
Ingram V M
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.8.4782
Published
1981-08-00
Pages
4782-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC320247
Subset
IM
Grants
NIADDK NIH HHS · AM 13945 · United States
Databases
GENBANK
J00862
External Links
PubMed source DOI: 10.1073/pnas.78.8.4782 PMC Full Text View journal details
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