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PMID: 6170978 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

alpha-Fetoprotein and albumin genes are in tandem in the mouse genome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 8 ·1981-08-00 ·Pages 4694-8

Ingram RS, Scott RW, Tilghman SM

Abstract

The urine alpha-fetoprotein (AFP) and serum albumin genes most probably arose in evolution as the consequence of a duplication of a common ancestral gene. They have both been previously mapped to chromosome 5 in the mouse. We now have evidence that these genes are closely linked. By using a unique copy DNA probe derived from previously cloned AFP 5' flanking DNA, a recombinant DNA phage has been isolated, from a bacteriophage DNA library, that contains sequences flanking the 5' end of the AFP gene and the 3' end of the albumin gene. Restriction endonuclease mapping and DNA sequence determination of the recombinant phage and comparison to total genomic DNA confirmed that the genes are in tandem, 13.5 kilobase pairs apart, with the albumin gene to the 5' side of the AFP gene. Thus, they are transcribed from the same strand of DNA.

MeSH Terms
Albumins/genetics Animals Bacteriophage lambda Chromosome Mapping Cloning, Molecular DNA, Recombinant Genes Genetic Linkage Mice/genetics Transcription, Genetic alpha-Fetoproteins/genetics
Chemicals
Albumins DNA, Recombinant alpha-Fetoproteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ingram R S
Scott R W
Tilghman S M
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46 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.8.4694
Published
1981-08-00
Pages
4694-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC320229
Subset
IM
Grants
NCI NIH HHS · CA 06927 · United States
NCI NIH HHS · CA 28050 · United States
NCRR NIH HHS · RR 05539 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.8.4694 PMC Full Text View journal details
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