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PMID: 6168921 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effect of capsaicin pretreatment on capsaicin-evoked release of immunoreactive somatostatin and substance P from primary sensory neurons.

Naunyn-Schmiedeberg's archives of pharmacology ·Vol. 316 ·No. 1 ·1981-02-00 ·Pages 38-41

Gamse R, Lackner D, Gamse G, Leeman SE

Abstract

Release of immunoreactive somatostatin (I-SRIF) and immunoreactive substance P (I-SP) was studied from slices prepared from upper dorsal horn (UDH) and lower dorsal plus ventral horn (LDH-VH) of rat spinal cord. Superfusion with capsaicin (10 microM) led to release of I-SRIF and I-SP from UDH slices but not from LDH-VH slices. The capsaicin-evoked release of I-SP was 6 fold higher than that of I-SRIF. A pulse of 60 mM K+ applied after the capsaicin pulse caused release of I-SRIF and I-SP from UDH as well as LDH-VH slices. Pretreatment of rats with capsaicin (125 mg/kg, s.c.) led to a nearly 40% depletion of I-SP in slices from UDH only. Capsaicin-evoked release from these slices was reduced by 81% for I-SRIF and by 79% for I-SP. Release evoked by K+ remained unchanged. These results indicate that capsaicin causes release of both I-SRIF and-I-SP and that this release is most likely restricted to primary sensory neurons. The marked reduction of the release of I-SP after systemic capsaicin pretreatment may well represent one of the, or even the reason for the insensitivity of capsaicin pretreated rats towards chemogenic pain.

MeSH Terms
Animals Capsaicin/pharmacology Fatty Acids, Unsaturated/pharmacology In Vitro Techniques Male Motor Neurons/drug effects Neurons/drug effects Potassium/pharmacology Rats Somatostatin/immunology,metabolism Spinal Cord/drug effects,metabolism Substance P/immunology,metabolism
Chemicals
Fatty Acids, Unsaturated Substance P Somatostatin Potassium Capsaicin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gamse R
Lackner D
Gamse G
Leeman S E
References (28)
28 references, click to expand
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Article Info
Journal
Naunyn-Schmiedeberg's archives of pharmacology
Abbr.
Naunyn Schmiedebergs Arch Pharmacol
ISSN
0028-1298
DOI
10.1007/BF00507224
Published
1981-02-00
Pages
38-41
Language
English
Region
Germany
NLM ID
0326264
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00507224 View journal details
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