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PMID: 6167986 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Partial characterization of gamma (immune) interferon mRNA extracted from human lymphocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 6 ·1981-06-00 ·Pages 3469-72

Taniguchi T, Pang RH, Yip YK, Henriksen D, Vilcek J

Abstract

gamma (immune) interferon (IFN-gamma) was induced in cultures of fresh human lymphocytes by combined treatment with a phorbol ester (12-O-tetradecanoylphorbol 13-acetate, TPA) and the T cell mitogen phytohemagglutinin (PHA). Compared to the IFN-gamma yields obtained with PHA induction alone, the inclusion of TPA caused a significant enhancement of IFN-gamma production. Poly(A)-containing mRNA was isolated from mononuclear cells induced with TPA and PHA. Injected into Xenopus laevis oocytes, this mRNA preparation gave rise to IFN activity with characteristic properties of human IFN-gamma. Sucrose density gradient centrifugation analysis showed that IFN-gamma mRNA sedimented at 15 S, suggesting that it contains a total of about 1400 nucleotides.

MeSH Terms
Animals Epitopes Female Humans Interferons/biosynthesis,genetics,immunology Lymphocytes/physiology Oocytes/physiology Phytohemagglutinins/pharmacology RNA, Messenger/genetics Tetradecanoylphorbol Acetate/pharmacology Xenopus laevis
Chemicals
Epitopes Phytohemagglutinins RNA, Messenger Interferons Tetradecanoylphorbol Acetate
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Taniguchi T
Pang R H
Yip Y K
Henriksen D
Vilcek J
References (18)
18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.6.3469
Published
1981-06-00
Pages
3469-72
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319590
Subset
IM
Grants
NIAID NIH HHS · AI-07057 · United States
NIAID NIH HHS · AI-12948 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.6.3469 PMC Full Text View journal details
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