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PMID: 6166249 Published · ppublish English Journal Article

Detection of Escherichia coli in foods: indole staining methods for cellulosic and polysulfone membrane filters.

Applied and environmental microbiology ·Vol. 41 ·No. 6 ·1981-06-00 ·Pages 1310-5

Sharpe AN, Peterkin PI, Rayman MK

Abstract

Optimized procedures for staining Escherichia coli colonies on cellulosic and polysulfone membrane filters are described. An explanation for the behavior of the Ehrlich reaction on membrane filters is suggested.

MeSH Terms
Bacteriological Techniques Cellulose Escherichia coli/isolation & purification Filtration/instrumentation Food Microbiology Indoles/analysis Polymers Staining and Labeling Sulfones Ultraviolet Rays
Chemicals
Indoles Polymers Sulfones Cellulose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sharpe A N
Peterkin P I
Rayman M K
References (4)
4 references, click to expand
  1. Enumeration of high numbers of bacteria using hydrophobic grid-membrane filters.
    Appl Microbiol. 1975 Oct;30(4):519-24 PMID: 1103728
  2. A rapid and direct plate method for enumerating Escherichia coli biotype I in food.
    J Appl Bacteriol. 1975 Oct;39(2):111-7 PMID: 1104553
  3. Improved detection of coliforms and Escherichia coli in foods by a membrane filter method.
    Appl Environ Microbiol. 1979 Sep;38(3):431-5 PMID: 394679
  4. ICMSF methods studies. XIII. An international comparative study of the MPN procedure and the Anderson-Baird-Parker direct plating method for the enumeration of Escherichia coli biotype I in raw meats.
    Can J Microbiol. 1979 Nov;25(11):1321-7 PMID: 396015
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.41.6.1310-1315.1981
Published
1981-06-00
Pages
1310-5
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC243916
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.41.6.1310-1315.1981 PMC Full Text View journal details
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