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PMID: 6166007 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Generation of fibroblast-like cells from cloned epithelial mammary cells in vitro: a possible new cell type.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 4 ·1981-04-00 ·Pages 2345-9

Dulbecco R, Henahan M, Bowman M, Okada S, Battifora H, Unger M

Abstract

We have isolated clonal cell lines from a nitrosomethylurea-induced mammary tumor in Wistar--Furth rats. The epithelial-like cells of these lines give rise to fibroblast-like cells. A study of several markers showed that the epithelial-like cells are similar to epithelial mammary cells. The fibroblastic cells, however, were similar to stromal cells, except for the production of collagen IV. The events occurring in these cultures are identical to those observed in previously isolated mammary cultures [Bennett, D. C., Peachey, L. A., Durbin, H. & Rudland, P. S. (1976) Cell 15, 283--298], suggesting their generality. The fibroblast-like cells generated in culture may be a hitherto unrecognized type of mammary cell.

MeSH Terms

Animals Antigens, Surface/metabolism Clone Cells/cytology Collagen/metabolism Epithelial Cells Female Fibroblasts/cytology Fibronectins/metabolism Fluorescent Antibody Technique Keratins/metabolism Mammary Glands, Animal/cytology Membrane Proteins/metabolism Muscle Proteins/metabolism Myosins/metabolism Neoplasms, Experimental/pathology Rats Thy-1 Antigens Vimentin

Chemicals

Antigens, Surface Fibronectins Membrane Proteins Muscle Proteins Thy-1 Antigens Vimentin Keratins Collagen Myosins

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Dulbecco R

Henahan M

Bowman M

Okada S

Battifora H

Unger M

References (11)

11 references, click to expand

Stardardized nomenclature for inbred strains of rats.
Transplantation. 1973 Sep;16(3):221-45 PMID: 4728822
N-nitrosomethylurea as mammary gland carcinogen in rats.
J Natl Cancer Inst. 1975 Feb;54(2):401-14 PMID: 1113323
Characteristics of rat normal mammary epithelial cells and dimethylbenzanthracene-induced mammary adenocarcinoma cells grown in monolayer culture.
In Vitro. 1974 Jul-Aug;10:51-62 PMID: 4220124
Comparison of the effects of hormones on DNA synthesis in cell cultures of nonneoplastic and neoplastic mammary epithelium from rats.
Cancer Res. 1977 Aug;37(8 Pt 1):2492-504 PMID: 872078
Immunofluorescent staining of keratin fibers in cultured cells.
Cell. 1978 Jul;14(3):469-76 PMID: 357009
Structurally distinct collagen types.
Annu Rev Biochem. 1980;49:957-1003 PMID: 6157354
Thy-1: a differentiation marker of potential mammary myoepithelial cells in vitro.
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6093-7 PMID: 366610
Differentiation of a rat mammary cell line in vitro.
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1256-60 PMID: 375233
Intermediate-sized filaments of human endothelial cells.
J Cell Biol. 1979 Jun;81(3):570-80 PMID: 379021
Intermediate-sized filaments of the prekeratin type in myoepithelial cells.
J Cell Biol. 1980 Mar;84(3):633-54 PMID: 6153658
A possible mammary stem cell line.
Cell. 1978 Sep;15(1):283-98 PMID: 699048

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.78.4.2345

Published

1981-04-00

Pages

2345-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC319342

Subset

Grants

NCI NIH HHS · 1-RO1 CA21993 · United States

External Links

PubMed source DOI: 10.1073/pnas.78.4.2345 PMC Full Text View journal details

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Generation of fibroblast-like cells from cloned epithelial mammary cells in vitro: a possible new cell type.

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