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PMID: 6165353 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Immunochemical distinction between two different chains of type IV collagen.

The Biochemical journal ·Vol. 191 ·No. 2 ·1980-11-01 ·Pages 517-22

Risteli J, Schuppan D, Glanville RW, Timpl R

Abstract

Antisera against mouse and human basement-membrane type IV collagen showed in radioimmunoassays distinct binding with large pepsin fragments obtained from the C-terminal portions of alpha 1 (IV)- and alpha 2 (IV)-chains. These reactions were specific for each constituent polypeptide chain. The data were confirmed by immunoadsorption, allowing the separation of antibodies with restricted chain specificity. Inhibition assays with CNBr peptides demonstrated the different localizations of antigenic determinants, which were either species-specific or shared by the human and mouse antigens.

MeSH Terms
Animals Antibody Specificity Antigen-Antibody Reactions Basement Membrane/analysis Collagen/immunology Cyanogen Bromide Epitopes/analysis Humans Mice Pepsin A Peptide Fragments/immunology Radioimmunoassay
Chemicals
Epitopes Peptide Fragments Collagen Pepsin A Cyanogen Bromide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Risteli J
Schuppan D
Glanville R W
Timpl R
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21 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1910517
Published
1980-11-01
Pages
517-22
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1162242
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1910517 PMC Full Text View journal details
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