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PMID: 6149743 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Amino acid oxidation and alanine production in rat hemidiaphragm in vitro. Effects of dichloroacetate.

The Biochemical journal ·Vol. 223 ·No. 1 ·1984-10-01 ·Pages 113-7

Palmer TN, Caldecourt MA, Sugden MC

Abstract

Dichloroacetate (an activator of pyruvate dehydrogenase) stimulates 14CO2 production from [U-14C]glucose, but not from [U-14C]glutamate, [U-14C]aspartate, [U-14C]- and [1-14C]-valine and [U-14C]- and [1-14C]-leucine. It is concluded (1) that pyruvate dehydrogenase is not rate-limiting in the oxidation to CO2 of amino acids that are metabolized to tricarboxylic acid-cycle intermediates, and (2) that carbohydrate (and not amino acids) is the main carbon precursor in alanine formation in muscle.

MeSH Terms
Acetates/pharmacology Alanine/metabolism Amino Acids/metabolism Animals Aspartic Acid/metabolism Diaphragm/metabolism Dichloroacetic Acid/pharmacology Glucose/metabolism Glutamates/metabolism Glutamic Acid In Vitro Techniques Male Muscles/drug effects,metabolism Oxidation-Reduction Rats Rats, Inbred Strains Starvation/metabolism
Chemicals
Acetates Amino Acids Glutamates Aspartic Acid Glutamic Acid Dichloroacetic Acid Glucose Alanine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Palmer T N
Caldecourt M A
Sugden M C
References (16)
16 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2230113
Published
1984-10-01
Pages
113-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1144271
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2230113 PMC Full Text View journal details
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