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PMID: 6146981 Published · ppublish English Journal Article

Epidermal growth factor inhibits the synthesis of the nuclear protein cyclin in A431 human carcinoma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 15 ·1984-08-00 ·Pages 4848-50

Bravo R

Abstract

The effect of epidermal growth factor (EGF) on the synthesis of the nuclear protein cyclin and its relationship with cell proliferation has been studied in human carcinoma A431 cells. Quantitative two-dimensional gel electrophoretic analysis of [35S]methionine-labeled polypeptides from EGF-treated and untreated A431 cells showed that the synthesis of cyclin (0.07% of total labeled protein) could be decreased to 20% (less than 0.01% of total labeled protein) by EGF at a concentration of 100 ng/ml. A good correlation between the effect of different concentrations of EGF on cyclin synthesis and A431 cell proliferation was found. A431-derived cells resistant to the growth-inhibitory effect of EGF showed no significant changes in cyclin synthesis after EGF treatment. Taken together, these results support the idea that cyclin may be an important protein that is involved in the control of cell proliferation.

MeSH Terms
Carcinoma/metabolism Cell Cycle Cell Nucleus/metabolism Cells, Cultured Drug Resistance Epidermal Growth Factor/pharmacology Humans Isoelectric Point Molecular Weight Neoplasm Proteins/biosynthesis Nucleoproteins/biosynthesis Proliferating Cell Nuclear Antigen
Chemicals
Neoplasm Proteins Nucleoproteins Proliferating Cell Nuclear Antigen Epidermal Growth Factor
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Bravo R
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.15.4848
Published
1984-08-00
Pages
4848-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391588
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.15.4848 PMC Full Text View journal details
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