Characterization of proliferating cell nuclear antigen recognized by autoantibodies in lupus sera.
A nuclear antigen associated with cell proliferation (proliferating cell nuclear antigen [PCNA]) and blast transformation is recognized by autoantibodies in the sera of some patients with systemic lupus erythematosus. Using this autoantibody as a reagent, PCNA was purified 120-fold by ammonium sulfate fractionation, DEAE chromatography, and Sephadex G200 gel filtration. The antigenicity of PCNA was sensitive to trypsin but resistant to ribonuclease and deoxyribonuclease, suggesting that the antigenic determinant resided in protein and not nucleic acids. PCNA was inactivated at 56 degrees C for 30 min. Isoelectrophoretic focusing showed that the pI was 4.8. Analysis of immunoprecipitates on polyacrylamide gels showed the presence of IgG heavy and light chains and a single polypeptide band of 33,000 mol wt. This polypeptide band was the reactive antigen in immunoblotting (Western transfer) assays.
17 references, click to expand
-
The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents.Immunochemistry. 1969 Jan;6(1):53-66 PMID: 4179831
-
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
-
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
-
Antinucleolar antibodies in human sera.J Immunol. 1973 Oct;111(4):996-1004 PMID: 4199560
-
Identification of antibodies to nuclear acidic antigens by counterimmunoelectrophoresis.Arthritis Rheum. 1976 May-Jun;19(3):574-80 PMID: 1084750
-
Autoantibody to a nuclear antigen in proliferating cells.J Immunol. 1978 Dec;121(6):2228-34 PMID: 102692
-
Changes in the synthesis of actin and other cell proteins after stimulation of serum-arrested cells.Proc Natl Acad Sci U S A. 1979 Mar;76(3):1298-302 PMID: 286312
-
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
-
Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.Proc Natl Acad Sci U S A. 1979 Nov;76(11):5495-9 PMID: 316537
-
Identification of a nuclear and of a cytoplasmic polypeptide whose relative proportions are sensitive to changes in the rate of cell proliferation.Exp Cell Res. 1981 Dec;136(2):311-9 PMID: 7308310
-
A nuclear antigen associated with cell proliferation and blast transformation.J Exp Med. 1981 Dec 1;154(6):1899-909 PMID: 6172535
-
Human proteins sensitive to neoplastic transformation in cultured epithelial and fibroblast cells.Clin Chem. 1982 Apr;28(4 Pt 2):949-54 PMID: 6280894
-
Protein antigens of the RNA-protein complexes detected by anti-SM and anti-RNP antibodies found in serum of patients with systemic lupus erythematosus and related disorders.J Exp Med. 1982 Nov 1;156(5):1475-85 PMID: 7130904
-
Autoantibodies to nuclear antigens (ANA): their immunobiology and medicine.Adv Immunol. 1982;33:167-240 PMID: 6182766
-
Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation.Int J Cancer. 1983 Jan 15;31(1):13-20 PMID: 6339421
-
Characterization by human autoantibody of a nuclear antigen related to the cell cycle.EMBO J. 1983;2(5):743-9 PMID: 6357785
-
Protein measurement with the Folin phenol reagent.J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
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