Effects of glnL and other regulatory loci on regulation of transcription of glnA-lacZ fusions in Klebsiella aerogenes.
Mutants of Klebsiella aerogenes containing genetic fusions of glnA to lacZ were isolated by using Mu dl (lac, bla) bacteriophage and a Mu Kmr helper phage with the host range of bacteriophage P1. Synthesis of beta-galactosidase in these strains is regulated in response to nitrogen metabolites and regulatory gln loci and is rendered constitutive by a mutation in the linked glnL gene. Complementation studies indicated that glnL is a separate locus from glnA and glnG and that insertions in glnA are partially polar on glnL expression. These results support the hypothesis that glnA, glnL, and glnG are organized in an operon with multiple promoters.
18 references, click to expand
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Genetic and metabolic control of enzymes responsible for histidine degradation in Salmonella typhimurium. 4-imidazolone-5-propionate amidohydrolase and N-formimino-L-glutamate formiminohydrolase.J Biol Chem. 1971 May 25;246(10):3320-9 PMID: 4930059
-
Glutamine synthetase and the regulation of histidase formation in Klebsiella aerogenes.J Biol Chem. 1973 Jun 25;248(12):4334-44 PMID: 4145798
-
Direct selection for P1-sensitive mutants of enteric bacteria.J Bacteriol. 1974 Jun;118(3):810-4 PMID: 4598005
-
Biochemical parameters of glutamine synthetase from Klebsiella aerogenes.J Bacteriol. 1977 Feb;129(2):1001-9 PMID: 14104
-
Autogenous regulation of the synthesis of glutamine synthetase in Klebsiella aerogenes.J Bacteriol. 1977 Oct;132(1):106-12 PMID: 21158
-
Involvement of the product of the glnF gene in the autogenous regulation of glutamine synthetase formation in Klebsiella aerogenes.J Bacteriol. 1978 Mar;133(3):1329-38 PMID: 25264
-
Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences.Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530-3 PMID: 159458
-
A new glnA-linked regulatory gene for glutamine synthetase in Escherichia coli.Proc Natl Acad Sci U S A. 1979 Sep;76(9):4544-8 PMID: 41243
-
Nitrogen control in Salmonella: regulation by the glnR and glnF gene products.Proc Natl Acad Sci U S A. 1979 Sep;76(9):4576-80 PMID: 41244
-
Inhibition of Escherichia coli exonuclease V by bacteriophage Mu.Virology. 1980 Apr 15;102(1):214-7 PMID: 6245503
-
Mutations in two unlinked genes are required to produce asparagine auxotrophy in Escherichia coli.J Bacteriol. 1980 Apr;142(1):221-8 PMID: 6102983
-
Regulation of the synthesis of glutamine synthetase by the PII protein in Klebsiella aerogenes.Proc Natl Acad Sci U S A. 1980 May;77(5):2636-40 PMID: 6104810
-
Infection of Salmonella typhimurium with coliphage Mu d1 (Apr lac): construction of pyr::lac gene fusions.J Bacteriol. 1981 Jan;145(1):299-305 PMID: 6450746
-
Regulation of expression from the glnA promoter of Escherichia coli in the absence of glutamine synthetase.Proc Natl Acad Sci U S A. 1980 Dec;77(12):7372-6 PMID: 6111793
-
Physical and genetic characterization of the glnA--glnG region of the Escherichia coli chromosome.Proc Natl Acad Sci U S A. 1981 Jun;78(6):3743-7 PMID: 6115384
-
Complex glnA-glnL-glnG operon of Escherichia coli.J Bacteriol. 1982 Apr;150(1):202-13 PMID: 6120929
-
Characterization of a gene, glnL, the product of which is involved in the regulation of nitrogen utilization in Escherichia coli.J Bacteriol. 1982 Apr;150(1):214-20 PMID: 6120930
-
Role of glnA-linked genes in regulation of glutamine synthetase and histidase formation in Klebsiella aerogenes.J Bacteriol. 1982 Apr;150(1):221-30 PMID: 6120931
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