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PMID: 6120203 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Leukotriene generation by eosinophils.

The Journal of experimental medicine ·Vol. 155 ·No. 2 ·1982-02-01 ·Pages 390-402

Jörg A, Henderson WR, Murphy RC, Klebanoff SJ

Abstract

Horse eosinophils purified to greater than 98% generated slow reacting substance (SRS) when incubated with the calcium ionophore A23187. On a per cell basis, eosinophils generated four to five times the SRS produced by similarly treated horse neutrophils. Eosinophil SRS production was inhibited by 5,8,11,14-eicosatetraynoic acid and augmented by indomethacin and arachidonic acid, suggesting that it was a product(s) of the lipoxygenase pathway of arachidonic acid metabolism. Compounds with SRS activity were purified by high-pressure liquid chromatography (HPLC) and identified by ultraviolet spectra, spectral shift on treatment with lipoxygenase, incorporation of [14C]arachidonic acid, gas chromatography-mass spectrometry, and comparison of retention times on HPLC to authentic standards. The eosinophil products characterized were 5-(S), 12-(R)-dihydroxy-6-cis-8, 10-trans-14-cis-eicosatetraenoic acid (leukotriene B4) and its 5-(S), 12-(R)-6-trans and 5-(S), 12-(S)-6-trans isomers, 5-(S)-hydroxy-6-(R)-S-glutathionyl-7,9-trans-11, 14-cis-eicosatetraenoic acid (leukotriene C4) and its 11-trans isomer, and 5-(S)-hydroxy-6-(R)-S-cysteinylglycine-7,9-trans-11,14-cis-eicosatetraenoic acid (leukotriene D4).

MeSH Terms
Animals Arachidonic Acids/biosynthesis,isolation & purification Autacoids/antagonists & inhibitors,biosynthesis,pharmacology Calcimycin/pharmacology Chromones/pharmacology Eosinophils/metabolism Guinea Pigs Horses Humans L-Lactate Dehydrogenase/metabolism Leukotriene B4 Mice Mice, Inbred Strains Neutrophils/metabolism SRS-A/biosynthesis,isolation & purification
Chemicals
Arachidonic Acids Autacoids Chromones SRS-A Leukotriene B4 Calcimycin FPL 55712 L-Lactate Dehydrogenase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jörg A
Henderson W R
Murphy R C
Klebanoff S J
References (32)
32 references, click to expand
  1. Selective inhibitor of slow reacting substance of anaphylaxis.
    Nat New Biol. 1973 Oct 17;245(146):215-7 PMID: 4147765
  2. Drugs which inhibit prostaglandin biosynthesis.
    Pharmacol Rev. 1974 Mar;26(1):33-67 PMID: 4208101
  3. Total eosinophil counts in the management of bronchial asthma.
    N Engl J Med. 1975 May 29;292(22):1152-5 PMID: 1124105
  4. Release of slow reacting substance of anaphylaxis (SRS-A) from human leukocytes by the calcium ionophore A23187.
    J Immunol. 1976 Jun;116(6):1677-81 PMID: 58045
  5. Release of slow reacting substance (SRS) from rat basophilic leukemia (RBL-1) cells.
    J Immunol. 1977 Aug;119(2):618-22 PMID: 328779
  6. Application and evaluation of limited mass monitoring with a gas chromatograph mass spectrometer computer system.
    Biomed Mass Spectrom. 1978 Jul;5(7):444-52 PMID: 678615
  7. A rapid and simple method for the isolation of pure eosinophilic leukocytes from horse blood.
    Experientia. 1978 Dec 15;34(12):1654-6 PMID: 729750
  8. Transformation of arachidonic acid by rabbit polymorphonuclear leukocytes. Formation of a novel dihydroxyeicosatetraenoic acid.
    J Biol Chem. 1979 Apr 25;254(8):2643-6 PMID: 429307
  9. Mast cell granule peroxidase: location, secretion, and SRS-A inactivation.
    J Immunol. 1979 Apr;122(4):1322-8 PMID: 87430
  10. Slow reacting substance (SRS) from ionophore A23187-stimulated peritoneal mast cells of the normal rat. I. Conditions of generation and initial characterization.
    J Immunol. 1979 May;122(5):2083-9 PMID: 87453
  11. Metabolism of arachidonic acid in polymorphonuclear leukocytes. Structural analysis of novel hydroxylated compounds.
    J Biol Chem. 1979 Aug 25;254(16):7865-9 PMID: 468794
  12. Leukotriene C: a slow-reacting substance from murine mastocytoma cells.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4275-9 PMID: 41240
  13. Structure of leukotriene C. Identification of the amino acid part.
    Biochem Biophys Res Commun. 1979 Dec 28;91(4):1266-72 PMID: 43155
  14. Leukotriene D: a slow reacting substance from rat basophilic leukemia cells.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):2014-7 PMID: 6103542
  15. Identification of leukotriene C-1 as a major component of slow-reacting substance from rat mononuclear cells.
    J Immunol. 1980 Jul;125(1):115-7 PMID: 6103909
  16. Effect of the 5-hydroperoxide of eicosatetraenoic acid and inhibitors of the lipoxygenase pathway on the formation of slow reacting substance by rat basophilic leukemia cells; direct evidence that slow reacting substance is a product of the lipoxygenase pathway.
    J Immunol. 1980 Jul;125(1):163-8 PMID: 6103910
  17. Slow reacting substances (SRSs): the structure identification of SRSs from rat basophil leukaemia (RBL-1) cells.
    Prostaglandins. 1980 Feb;19(2):185-201 PMID: 6104346
  18. Identification of a component of rat mononuclear cell SRS as leukotriene D.
    Biochem Biophys Res Commun. 1980 Apr 29;93(4):1121-6 PMID: 6104957
  19. 11-Trans leukotriene C: a naturally occurring slow reacting substance.
    Biochem Biophys Res Commun. 1980 Jun 30;94(4):1133-9 PMID: 6772184
  20. Leukotriene B, a potent chemokinetic and aggregating substance released from polymorphonuclear leukocytes.
    Nature. 1980 Jul 17;286(5770):264-5 PMID: 6250050
  21. Slow reacting substances of anaphylaxis: identification of leukotrienes C-1 and D from human and rat sources.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3710-4 PMID: 6106193
  22. Dynamics of leukotriene C production by macrophages.
    J Exp Med. 1980 Nov 1;152(5):1236-47 PMID: 6107327
  23. Comparative airway and vascular activities of leukotrienes C-1 and D in vivo and in vitro.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4354-8 PMID: 6933488
  24. Mouse peritoneal macrophages release leukotriene C in response to a phagocytic stimulus.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4928-32 PMID: 6933538
  25. Leukotrienes and slow reacting substance of anaphylaxis (SRS-A).
    Allergy. 1980 Jul;35(5):375-81 PMID: 6778241
  26. Characterization of slow reacting substances (SRSs) of rat basophilic leukemia (RBL-1) cells: effect of cysteine on SRS profile.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6481-5 PMID: 6109281
  27. The action of leukotriene B4 (LTB4) on the lung.
    Prostaglandins Med. 1980 Dec;5(6):429-44 PMID: 6258182
  28. Sequential conversion of the glutathionyl side chain of slow reacting substance (SRS) to cysteinyl-glycine and cysteine in rat basophilic leukemia cells stimulated with A-23187.
    Prostaglandins. 1980 Nov;20(5):863-86 PMID: 6110221
  29. From slow reacting substance of anaphylaxis (SRS-A) to leukotriene D4 (LTD4).
    Int J Immunopharmacol. 1980;2(4):281-93 PMID: 6782030
  30. The effect of leukotrienes C4 and D4 on the microvasculature of guinea-pig skin.
    Prostaglandins. 1981 Feb;21(2):315-21 PMID: 6894335
  31. Novel structural determinants of the human neutrophil chemotactic activity of leukotriene B.
    J Exp Med. 1981 Feb 1;153(2):482-7 PMID: 6264017
  32. Electrophysiological response of cerebellar Purkinje neurons to leukotriene D4 and B4.
    J Pharmacol Exp Ther. 1981 Oct;219(1):91-6 PMID: 6270301
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.155.2.390
Published
1982-02-01
Pages
390-402
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2186605
Subset
IM
Grants
NIAID NIH HHS · AI07763 · United States
NIAID NIH HHS · AI17758 · United States
NHLBI NIH HHS · HL25785 · United States
External Links
PubMed source DOI: 10.1084/jem.155.2.390 PMC Full Text View journal details
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