Abstract
The enzymatic activity of glutamine synthetase [GS; L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2] from the Gram-positive bacterium Streptomyces cattleya is regulated by covalent modification. In whole cells containing high levels of GS the addition of ammonium chloride leads to a rapid decline in GS activity. Crude extracts prepared from such ammonia-shocked cells had very low levels of GS activity as measured by biosynthetic and gamma-glutamyltransferase assays. Incubation of the crude extracts with snake venom phosphodiesterase restored GS activity. In cell extracts, GS was also inactivated by an ATP- and glutamine-dependent reaction. Radioactive labeling studies demonstrated the incorporation of an AmP moiety into GS protein upon modification. Our results suggest a covalent modification of GS in a Gram-positive bacterium. This modification appears to be adenylylation of the GS subunit similar to that found in the Gram-negative bacteria.
MeSH Terms
Adenine/metabolism
Adenosine Triphosphate/metabolism
Ammonium Chloride/pharmacology
Glutamate-Ammonia Ligase/antagonists & inhibitors,metabolism
Glutamine/metabolism
Isoenzymes/pharmacology
Phosphodiesterase I
Phosphoric Diester Hydrolases/pharmacology
Streptomyces/drug effects,enzymology
Chemicals
Isoenzymes
Ammonium Chloride
Glutamine
Adenosine Triphosphate
Phosphoric Diester Hydrolases
Phosphodiesterase I
phosphodiesterase I, snake venom
Glutamate-Ammonia Ligase
Adenine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Streicher S L
Tyler B
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16 references, click to expand
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