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PMID: 6109282 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of two Escherichia coli factor Y effector sites near the origins of replication of the plasmids (ColE1 and pBR322.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 11 ·1980-11-00 ·Pages 6521-5

Zipursky SL, Marians KJ

Abstract

The Escherichia coli replication factor Y has been characterized as a phi X174 (+) strand specific DNA-dependent phosphohydrolase. In conjunction with other E. coli replication proteins, factor Y is involved in the formation of heterogeneous primers that are elongated by the E. coli DNA polymerase III elongation machinery. We report here that the heat-denatured DNAs of plasmids pBR322 and ColE1 serve as effectors for the hydrolysis of ATP by factor Y. The DNA sequences of pBR322 responsible for factor Y effector activity have been localized. Two separate regions of the pBR322 chromosome support Y ATPase activity. These sequences are near the replication origin and are located on opposite DNA strands.

MeSH Terms
Adenosine Triphosphatases/metabolism Bacterial Proteins/metabolism Bacteriocin Plasmids Binding Sites DNA Helicases/metabolism DNA Replication Plasmids Substrate Specificity
Chemicals
Bacterial Proteins Adenosine Triphosphatases DNA Helicases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zipursky S L
Marians K J
References (24)
24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.11.6521
Published
1980-11-00
Pages
6521-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350317
Subset
IM
Grants
NIGMS NIH HHS · 1R01 GM26410 · United States
NIGMS NIH HHS · 5R01 GM13344 · United States
NIGMS NIH HHS · GM07491-04 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.11.6521 PMC Full Text View journal details
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