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PMID: 6097799 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning of cybB, the gene for cytochrome b561 of Escherichia coli K12.

Molecular & general genetics : MGG ·Vol. 198 ·No. 2 ·1984-00-00 ·Pages 1-6

Murakami H, Kita K, Anraku Y

Abstract

A 37 kb fragment of DNA from an F-prime factor, F100-12, which showed a gene dosage effect on b-type cytochromes, was cloned with a cosmid vector, pHC79. Gel filtration of cytochromes and product analysis of the hybrid plasmids indicated that this fragment contained cybB, the structural gene for cytochrome b561. A chromosomal DNA fragment carrying the cybB gene was cloned by the plaque hybridization technique with Charon 4A as a vector. The gene was subcloned into pBR322 and was located in a 1.3 kb DNA fragment. It was concluded that the cybB gene is located on the chromosome of Escherichia coli K12.

MeSH Terms
Chromosome Mapping Cloning, Molecular Cytochrome b Group/genetics Escherichia coli/genetics Genes Genes, Bacterial Genetic Vectors Plasmids
Chemicals
Cytochrome b Group cytochrome b561
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Murakami H
Kita K
Anraku Y
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00328692
Published
1984-00-00
Pages
1-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00328692
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