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PMID: 6096861 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Polymorphisms of a human variable heavy chain gene show linkage with constant heavy chain genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 24 ·1984-12-00 ·Pages 7840-4

Johnson MJ, Natali AM, Cann HM, Honjo T, Cavalli-Sforza LL

Abstract

In this study, restriction fragment length polymorphisms (RFLPs) were identified with an immunoglobulin variable heavy chain region (Ig VH) probe and the inheritance of the polymorphisms was analyzed in families. Linkage within the VHII gene cluster and between the VHII and Ig CH genes was investigated by lod (logarithm of odds) score analysis. In addition, the position of the VHII genes was determined in relation to another polymorphic locus--D14S1, which is tightly linked and centromeric to the CH genes. Genetic associations between genes in the CH and VH clusters were analyzed. These RFLPs represent genetically characterized VH region polymorphisms and it is hoped that they will facilitate the study of disease correlations as well as further the understanding of the genetics of the immunoglobulin heavy chain genes in humans.

MeSH Terms
Base Composition Base Sequence DNA Restriction Enzymes Genes Genetic Linkage Genotype Humans Immunoglobulin Constant Regions/genetics Immunoglobulin Heavy Chains/genetics Immunoglobulin Variable Region/genetics Immunoglobulins/genetics Molecular Weight Polymorphism, Genetic
Chemicals
Immunoglobulin Constant Regions Immunoglobulin Heavy Chains Immunoglobulin Variable Region Immunoglobulins DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Johnson M J
Natali A M
Cann H M
Honjo T
Cavalli-Sforza L L
References (43)
43 references, click to expand
  1. Experimental approaches tohomogenous antibody populations. iIndividual antigenic specificity, cross specificity and diversity of human antibodies.
    Fed Proc. 1970 Jan-Feb;29(1):55-8 PMID: 4983814
  2. Report of the Committee on Human Gene Mapping by Recombinant DNA Techniques.
    Cytogenet Cell Genet. 1984;37(1-4):210-73 PMID: 6360559
  3. Genetics of the antibody response to dextran in mice.
    Science. 1972 Jul 14;177(4044):178-80 PMID: 4114394
  4. Evidence for the linkage of the IGC H locus to a gene controlling the idiotypic specificity of anti-p-azophenylarsonate antibodies in strain A mice.
    J Exp Med. 1973 Jan 1;137(1):22-31 PMID: 4120094
  5. Localization of idiotypic antigenic determinants in the Fv region of murine myeloma protein MOPC-315.
    Proc Natl Acad Sci U S A. 1973 May;70(5):1585-7 PMID: 4123072
  6. Estimation of the recombination fraction in human pedigrees: efficient computation of the likelihood for human linkage studies.
    Am J Hum Genet. 1974 Sep;26(5):588-97 PMID: 4422075
  7. Selection in complex genetic systems III. An effect of allele multiplicity with two loci.
    Genetics. 1975 Feb;79(2):333-47 PMID: 1132683
  8. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  9. Inherited immunoglobulin idiotypes of the mouse.
    Immunol Rev. 1977;34:119-38 PMID: 66781
  10. Crossing-over frequency in the Igh region of the mouse genome.
    Ann Immunol (Paris). 1984 Jan-Feb;135C(1):169-73 PMID: 6424551
  11. Molecular basis of a mouse strain-specific anti-hapten response.
    Cell. 1983 May;33(1):85-93 PMID: 6432337
  12. Rearranged immunoglobulin heavy chain variable region (VH) pseudogene that deletes the second complementarity-determining region.
    Proc Natl Acad Sci U S A. 1984 Aug;81(16):5194-8 PMID: 6089186
  13. Allotypy in rabbit 19S protein.
    Biochem Biophys Res Commun. 1963 May 3;11:170-5 PMID: 13985246
  14. Sequential tests for the detection of linkage.
    Am J Hum Genet. 1955 Sep;7(3):277-318 PMID: 13258560
  15. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  16. Hv(1), a variable-region genetic marker of human immunoglobulin heavy chains.
    Science. 1978 Apr 21;200(4339):327-9 PMID: 416494
  17. A complete immunoglobulin gene is created by somatic recombination.
    Cell. 1978 Sep;15(1):1-14 PMID: 100225
  18. The integrated genome of murine leukemia virus.
    Cell. 1978 Nov;15(3):1003-10 PMID: 728982
  19. Exon shuffling generates an immunoglobulin heavy chain gene.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):2138-42 PMID: 6769120
  20. An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH.
    Cell. 1980 Apr;19(4):981-92 PMID: 6769593
  21. Linkage relationship between variable and constant region allotypic determinants of human immunoglobulin heavy chains.
    Nature. 1980 Jul 24;286(5771):406-7 PMID: 6772963
  22. Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.
    Nature. 1980 Aug 14;286(5774):676-83 PMID: 6774258
  23. Structure and multiplicity of genes for the human immunoglobulin heavy chain variable region.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6561-5 PMID: 6450418
  24. A highly polymorphic locus in human DNA.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6754-8 PMID: 6935681
  25. Cloning of human immunoglobulin mu gene and comparison with mouse mu gene.
    Nucleic Acids Res. 1980 Dec 20;8(24):5983-91 PMID: 6450943
  26. Heavy chain variable region contribution to the NPb family of antibodies: somatic mutation evident in a gamma 2a variable region.
    Cell. 1981 Jun;24(3):625-37 PMID: 6788376
  27. Diversity of germ-line immunoglobulin VH genes.
    Nature. 1981 Jul 30;292(5822):426-30 PMID: 6789211
  28. Human antibody genes: V gene variability and CH gene switching strategies.
    Immunol Rev. 1981;59:69-91 PMID: 6273296
  29. Polymorphism of germ-line immunoglobulin VH genes correlates with allotype and idiotype markers.
    Eur J Immunol. 1981 Dec;11(12):1017-20 PMID: 6276186
  30. Human immunoglobulin heavy chain genes map to a region of translocations in malignant B lymphocytes.
    Science. 1982 Apr 16;216(4543):301-3 PMID: 6801764
  31. Immunoglobulin heavy chain genes in humans are located on chromosome.
    Ann Hum Genet. 1981 Oct;45(Pt 4):331-5 PMID: 6805411
  32. An immunoglobulin VH pseudogene.
    J Mol Appl Genet. 1981;1(2):93-101 PMID: 6286821
  33. Organization and evolution of immunoglobulin VH gene subgroups.
    Proc Natl Acad Sci U S A. 1982 Jul;79(14):4405-9 PMID: 6812048
  34. Translocation of immunoglobulin VH genes in Burkitt lymphoma.
    Proc Natl Acad Sci U S A. 1982 Sep;79(18):5611-5 PMID: 6813863
  35. Highly polymorphic DNA site D14S1 maps to the region of Burkitt lymphoma translocation and is closely linked to the heavy chain gamma 1 immunoglobulin locus.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7395-9 PMID: 6818543
  36. The genetic basis of antibody production: the dominant anti-arsonate idiotype response of the strain A mouse.
    Eur J Immunol. 1982 Dec;12(12):1023-32 PMID: 6186498
  37. Transcriptional activation of the translocated c-myc oncogene in burkitt lymphoma.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):820-4 PMID: 6402776
  38. Evolutionary aspects of immunoglobulin heavy chain variable region (VH) gene subgroups.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):855-9 PMID: 6298778
  39. Multiple DNA fragment polymorphisms associated with immunoglobulin mu chain switch-like regions in man.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):467-71 PMID: 6300846
  40. Analysis of a break in chromosome 14 mapping to the region of the immunoglobulin heavy chain locus.
    Proc Natl Acad Sci U S A. 1983 Apr;80(7):1997-2001 PMID: 6403943
  41. Conservation and divergence of immunoglobulin VH pseudogenes.
    EMBO J. 1983;2(10):1795-800 PMID: 6315403
  42. Transcriptional activation of an unrearranged and untranslocated c-myc oncogene by translocation of a C lambda locus in Burkitt.
    Proc Natl Acad Sci U S A. 1983 Nov;80(22):6922-6 PMID: 6417658
  43. Common individual antigenic determinants in five of eight BALB-c IgA myeloma proteins that bind phosphoryl choline.
    J Exp Med. 1970 Oct 1;132(4):737-51 PMID: 4101363
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.24.7840
Published
1984-12-00
Pages
7840-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392248
Subset
IM
Grants
NIGMS NIH HHS · GM 07790 · United States
NIGMS NIH HHS · GM 28428 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.24.7840 PMC Full Text View journal details
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