Home LiteratureArticle Details

PMID: 6096087 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Activity of DNase associated with replication of Epstein-Barr virus in NPC-204 cells.

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology ·Vol. 17 ·No. 3 ·1984-08-00 ·Pages 121-30

Liu MY, Chen JY, Yang CS

Abstract

A deoxyribonuclease derived from Epstein-Barr virus (EBV)-producing lymphoblastoid cell line (NPC-204 cells) treated with IUdR was purified by DEAE cellulose, phospho-cellulose, and DNA-cellulose columns with 45% recovery. The activity of the DNase was neutralized by serum of patient with nasopharyngeal carcinoma (NPC). The DNase had a strong requirement for divalent cations and an alkaline pH optimum. Its activity was inhibited by highly ionic strength and polyamines.

MeSH Terms

Cell Line Deoxyribonucleases/analysis,antagonists & inhibitors Enzyme Induction Herpesvirus 4, Human/enzymology,physiology Humans Hydrogen-Ion Concentration Idoxuridine/pharmacology Manganese/pharmacology Nasopharyngeal Neoplasms/enzymology Virus Replication

Chemicals

Manganese Deoxyribonucleases Idoxuridine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Liu M Y

Chen J Y

Yang C S

Article Info

Journal

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology

Abbr.

Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi

ISSN

0253-2662

Published

1984-08-00

Pages

121-30

Language

English

Region

China (Republic : 1949- )

NLM ID

8008067

Subset

External Links

PubMed source

Analysis Services

Activity of DNase associated with replication of Epstein-Barr virus in NPC-204 cells.

Contact

WeChat Official Account