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PMID: 6094980 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Streptomycin-sensitivity in Streptomyces glaucescens is due to deletions comprising the structural gene coding for a specific phosphotransferase.

Molecular & general genetics : MGG ·Vol. 196 ·No. 3 ·1984-00-00 ·Pages 513-20

Hintermann G, Crameri R, Vögtli M, Hütter R

Abstract

The wild type strain of Streptomyces glaucescens produces hydroxystreptomycin and has a natural resistance towards the streptomycin group aminoglycoside antibiotics. The inherent resistance is a genetically unstable character and mutant strains sensitive to streptomycins arise spontaneously at unusually high frequencies. The gene conferring streptomycin resistance was cloned and characterised as a streptomycin specific phosphotransferase. Hybridisation experiments show that the mutational event leading to sensitivity is due to large deletions, most likely on the chromosome, comprehending the structural gene coding for a streptomycin phosphotransferase and its flanking regions. Interspecific expression of the S. glaucescens phosphotransferase was found in Streptomyces lividans as well as in Escherichia coli.

MeSH Terms

Chromosome Deletion Cloning, Molecular DNA Restriction Enzymes Genes Genes, Bacterial Genotype Phosphotransferases/genetics Phosphotransferases (Alcohol Group Acceptor) Plasmids Species Specificity Streptomyces/drug effects,enzymology,genetics Streptomycin/toxicity

Chemicals

Phosphotransferases Phosphotransferases (Alcohol Group Acceptor) streptomycin 3''-kinase DNA Restriction Enzymes Streptomycin

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Hintermann G

Crameri R

Vögtli M

Hütter R

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25 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00436201

Published

1984-00-00

Pages

513-20

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00436201

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Streptomycin-sensitivity in Streptomyces glaucescens is due to deletions comprising the structural gene coding for a specific phosphotransferase.

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