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PMID: 6090414 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Proline uptake through the major transport system of Salmonella typhimurium is coupled to sodium ions.

Journal of bacteriology ·Vol. 160 ·No. 1 ·1984-10-00 ·Pages 22-7

Cairney J, Higgins CF, Booth IR

Abstract

Strains of Salmonella typhimurium deficient in one or more of the proline transport systems have been constructed and used to study the mechanism of energy coupling to transport. Proline uptake through the major proline permease (PP-I, putP) is shown to be absolutely coupled to Na+ ions and not to H+ ions as has previously been assumed. Transport through the minor proline permease (PP-II, proP), however, is unaffected by the presence or absence of Na+. The effect of Na+ on the kinetics of proline uptake shows that external Na+ increases the Vmax for transport. It seems probable that proline transport through PP-I is also coupled to Na+ ions in Escherichia coli.

MeSH Terms
Amino Acid Transport Systems, Neutral Biological Transport/drug effects DNA Transposable Elements Kinetics Membrane Transport Proteins/genetics,metabolism Potassium/pharmacology Proline/metabolism Salmonella typhimurium/enzymology,genetics Sodium/metabolism Species Specificity Transduction, Genetic
Chemicals
Amino Acid Transport Systems, Neutral DNA Transposable Elements Membrane Transport Proteins proline transporter Proline Sodium Potassium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cairney J
Higgins C F
Booth I R
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25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.160.1.22-27.1984
Published
1984-10-00
Pages
22-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214675
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.160.1.22-27.1984 PMC Full Text View journal details
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