The Na+-dependent regulation of the internal pH in chick skeletal muscle cells. The role of the Na+/H+ exchange system and its dependence on internal pH.
The internal pH (pHi) of chick muscle cells is determined by the transmembrane Na+ gradient. Li+, but not K+, Rb+ or Cs+, can substitute for Na+ for regulating the internal pH of chick muscle cells. Pharmacological evidence using amiloride and amiloride analogs has shown that the Na+/H+ exchange system is the membrane mechanism that couples the pHi to the transmembrane Na+ gradient. The pHi dependence of the amiloride-sensitive Na+/H+ exchange mechanism was defined. Internal H+ interacts cooperatively with the Na+/H+ exchange system, in contrast with external H+, thus indicating an asymmetrical behaviour of this exchanger. The half-maximum effect for the activation by the internal H+ of the Na+ transporting activity of the amiloride-sensitive Na+/H+ exchange was observed at pH 7.4. The Hill coefficient of the H+ concentration dependence is higher than 3. Insulin was shown to have no effect on the pHi of chick muscle cells.
27 references, click to expand
-
Determination of protein: a modification of the Lowry method that gives a linear photometric response.Anal Biochem. 1972 Aug;48(2):422-7 PMID: 4115981
-
An investigation of the ionic mechanism of intracellular pH regulation in mouse soleus muscle fibres.J Physiol. 1977 Dec;273(1):295-316 PMID: 23428
-
Ontogeny of insulin binding during chick skeletal myogenesis in vitro.Dev Biol. 1979 Feb;68(2):546-56 PMID: 437339
-
Intracellular pH measurements in Ehrlich ascites tumor cells utilizing spectroscopic probes generated in situ.Biochemistry. 1979 May 29;18(11):2210-8 PMID: 36128
-
Ionophores.Methods Enzymol. 1979;55:435-54 PMID: 379502
-
Uptake of 22Na+ by cultured dog kidney cells (MDCK).J Biol Chem. 1979 Nov 25;254(22):11431-9 PMID: 500650
-
pH regulation in barnacle muscle fibers: dependence on extracellular sodium and bicarbonate.Am J Physiol. 1981 Jan;240(1):C80-9 PMID: 6257119
-
Intracellular pH.Physiol Rev. 1981 Apr;61(2):296-434 PMID: 7012859
-
Stimulation of Na:H exchange by insulin.Biophys J. 1981 Feb;33(2):203-10 PMID: 7013841
-
Intracellular pH of sea urchin eggs measured by the dimethyloxazolidinedione (DMO) method.J Cell Biol. 1981 May;89(2):284-91 PMID: 7195903
-
Purification and pharmacological properties of eight sea anemone toxins from Anemonia sulcata, Anthopleura xanthogrammica, Stoichactis giganteus, and Actinodendron plumosum.Biochemistry. 1981 Sep 1;20(18):5245-52 PMID: 6117312
-
The appearance of voltage-sensitive Na+ channels during the in vitro differentiation of embryonic chick skeletal muscle cells.J Biol Chem. 1981 Dec 10;256(23):12355-61 PMID: 6271783
-
Sodium/proton exchange in mouse neuroblastoma cells.J Biol Chem. 1981 Dec 25;256(24):12883-7 PMID: 7309738
-
(Na+,K+)-cotransport in the Madin-Darby canine kidney cell line. Kinetic characterization of the interaction between Na+ and K+.J Biol Chem. 1982 Mar 10;257(5):2254-9 PMID: 6277889
-
Ontogeny of the (Na+,K+)-ATPase during chick skeletal myogenesis.J Biol Chem. 1982 May 25;257(10):5380-4 PMID: 6279598
-
The amiloride-sensitive Na+/H+ exchange system in skeletal muscle cells in culture.J Biol Chem. 1982 Aug 25;257(16):9394-400 PMID: 6286621
-
Growth factor activation of an amiloride-sensitive Na+/H+ exchange system in quiescent fibroblasts: coupling to ribosomal protein S6 phosphorylation.Proc Natl Acad Sci U S A. 1982 Jul;79(13):3935-9 PMID: 6287453
-
Elevation of the intracellular pH activates respiration and motility of sperm of the sea urchin, Strongylocentrotus purpuratus.J Biol Chem. 1982 Dec 25;257(24):14881-90 PMID: 7174670
-
Na+/H+ antiport in Swiss 3T3 cells: mitogenic stimulation leads to cytoplasmic alkalinization.Proc Natl Acad Sci U S A. 1982 Dec;79(24):7778-82 PMID: 6961450
-
Changes in internal pH associated with initiation of motility and acrosome reaction of sea urchin sperm.Dev Biol. 1983 Jan;95(1):31-45 PMID: 6825930
-
Intracellular pH and the control of cell differentiation in Dictyostelium discoideum.Nature. 1983 May 19-25;303(5914):244-5 PMID: 6843673
-
The amiloride-sensitive Na+/H+ antiport in 3T3 fibroblasts.J Biol Chem. 1983 May 25;258(10):6272-6 PMID: 6304031
-
Examination of the intracellular ionic environment and of ionophore action by null point measurements employing the fluorescein chromophore.J Biol Chem. 1983 May 25;258(10):6380-9 PMID: 6853488
-
Na+/H+ exchange and cytoplasmic pH in the action of growth factors in human fibroblasts.Nature. 1983 Aug 18-24;304(5927):645-8 PMID: 6410286
-
Ethylisopropyl-amiloride: a new and highly potent derivative of amiloride for the inhibition of the Na+/H+ exchange system in various cell types.Biochem Biophys Res Commun. 1983 Oct 14;116(1):86-90 PMID: 6315011
-
Structure-activity relationships of amiloride and certain of its analogues in relation to the blockade of the Na+/H+ exchange system.Mol Pharmacol. 1984 Jan;25(1):131-6 PMID: 6323947
-
Growth factor action and intracellular pH regulation in fibroblasts. Evidence for a major role of the Na+/H+ antiport.J Biol Chem. 1984 May 10;259(9):5809-15 PMID: 6325450
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