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PMID: 6089199 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of a biologically active fragment of human IgE epsilon chain in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 17 ·1984-09-00 ·Pages 5369-73

Liu FT, Albrandt KA, Bry CG, Ishizaka T

Abstract

cDNA corresponding to human IgE heavy (epsilon) chain mRNA was cloned from human IgE-secreting myeloma U266 cells. Partial nucleotide sequence analysis demonstrated that the cloned cDNA contained the coding region for about two-thirds of the CH2 and all of the CH3 and CH4 domains as well as the 3'-untranslated region. This epsilon cDNA was inserted into expression vector pUC7 and expression of an epsilon-chain fragment in Escherichia coli was demonstrated by protein blot analysis using 125I-labeled goat anti-human IgE as probe. The expression product was purified on a column of goat anti-human IgE-conjugated Sepharose 4B and the polypeptide was found to retain binding activity to human basophils.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Line Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Escherichia coli/genetics Humans Immunoglobulin E/genetics Immunoglobulin Fc Fragments/genetics Immunoglobulin Heavy Chains/genetics Immunoglobulin epsilon-Chains/genetics Multiple Myeloma/immunology
Chemicals
Immunoglobulin Fc Fragments Immunoglobulin Heavy Chains Immunoglobulin epsilon-Chains Immunoglobulin E DNA DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Liu F T
Albrandt K A
Bry C G
Ishizaka T
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.17.5369
Published
1984-09-00
Pages
5369-73
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391705
Subset
IM
Grants
NIAID NIH HHS · AI-19476 · United States
NIAID NIH HHS · AI-19477 · United States
NIAID NIH HHS · AI-19747 · United States
Databases
GENBANK
J00227, L00022, V00555
External Links
PubMed source DOI: 10.1073/pnas.81.17.5369 PMC Full Text View journal details
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