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PMID: 6088555 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Effects of cAMP on single cell motility in Dictyostelium.

The Journal of cell biology ·Vol. 99 ·No. 3 ·1984-09-00 ·Pages 1151-5

Varnum B, Soll DR

Abstract

The motility of individual, aggregation-competent amebae of Dictyostelium has been analyzed at different concentrations of cAMP under both nongradient and gradient conditions. The following is demonstrated: (a) concentrations of cAMP greater than 10(-8) M inhibit motility in a concentration-dependent fashion, decrease the frequency but not the degree of turning, and cause rounding in cell shape; (b) no concentration of cAMP stimulates motility, or positive chemokinesis; (c) concentrations of cAMP that stimulate a maximal chemotactic response do not affect motility and concentrations of cAMP that maximally inhibit motility do not stimulate chemotaxis under gradient conditions; and (d) the concentrations of cAMP that inhibit motility are identical under gradient and nongradient conditions.

MeSH Terms
Cell Movement/drug effects Chemotaxis/drug effects Cyclic AMP/pharmacology Dictyostelium/drug effects,physiology,ultrastructure Kinetics
Chemicals
Cyclic AMP
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Varnum B
Soll D R
References (21)
21 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.99.3.1151
Published
1984-09-00
Pages
1151-5
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113391
Subset
IM
Grants
NCI NIH HHS · CA28848 · United States
NIGMS NIH HHS · GM07228 · United States
NIGMS NIH HHS · GM25832 · United States
External Links
PubMed source DOI: 10.1083/jcb.99.3.1151 PMC Full Text View journal details
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