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PMID: 6086259 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Restriction site bank vectors. II. DNA sequence analysis of plasmid pJRD158.

DNA (Mary Ann Liebert, Inc.) ·Vol. 3 ·No. 3 ·1984-06-00 ·Pages 259-68

Heusterspreute M, Davison J

Abstract

pJRD158 is a small plasmid vector (3903 bp) derived from pBR327 and specifying resistance to ampicillin and tetracycline. It contains 28 unique restriction sites (and 4 nonunique restriction sites) that can be used for cloning. The DNA sequence and computer-assisted restriction site analysis of pJRD158 are reported. Evidence is also presented that suggests a 2-bp revision of the DNA sequence of pBR322 in the RNA primer region.

MeSH Terms

Ampicillin/toxicity Base Sequence DNA Restriction Enzymes Escherichia coli/drug effects Genetic Vectors Penicillin Resistance R Factors Tetracycline/toxicity

Chemicals

Ampicillin DNA Restriction Enzymes Tetracycline

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Heusterspreute M

Davison J

Article Info

Journal

DNA (Mary Ann Liebert, Inc.)

Abbr.

DNA

ISSN

0198-0238

DOI

10.1089/dna.1.1984.3.259

Published

1984-06-00

Pages

259-68

Language

English

Region

United States

NLM ID

8302432

Subset

Databases

GENBANK

J01749, K00005, M10282, M10283, M10286, M10356, M10784, M10785, M10786, M12802

External Links

PubMed source DOI: 10.1089/dna.1.1984.3.259

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Restriction site bank vectors. II. DNA sequence analysis of plasmid pJRD158.

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