The uptake and digestion of iodinated human serum albumin by macrophages in vitro.
Mouse peritoneal macrophages take up I*-HSA from their medium during in vitro cultivation. Conditions which promote I*-HSA uptake are the same as those which stimulate formation of pinocytic vesicles. Autoradiography of cells pulsed with (125)I-HSA showed that intracellular isotope is localized in perinuclear granules, or secondary lysosomes. Following a pulse of (125)I-HSA, intracellular radioactivity decreases and the amount of TCA-soluble isotope in the medium increases correspondingly. About 50% of the intracellular isotope is lost in 5 hr. The release of isotope from pulsed cells is not inhibited by parafluorophenylalanine, 2,4-dinitrophenol or by a reduction of the serum concentration of the medium. However, the processing of ingested (125)I-HSA is reversibly inhibited by reduced temperature. The TCA-soluble radioactive material excreted by pulsed macrophages was identified as monoiodotyrosine.
26 references, click to expand
-
Studies on the pathway of protein synthesis in tissue culture.J Biol Chem. 1953 May;202(1):273-89 PMID: 13061454
-
Studies on the fate of antigens in vitro. I. The effect of specific antibody on the fate of I 131 trace labeled human serum albumin in vitro in the presence of guinea pig monocytes.J Immunol. 1959 Apr;82(4):332-9 PMID: 13641668
-
Incorporation of Heterologous Deoxyribonucleic Acid into Mammalian Cells.Science. 1961 Feb 10;133(3450):381-2 PMID: 17815829
-
The in vitro differentiation of mononuclear phagocytes. IV. The ultrastructure of macrophage differentiation in the peritoneal cavity and in culture.J Exp Med. 1966 Apr 1;123(4):747-56 PMID: 5931921
-
The fate of bacteria within phagocytic cells. I. The degradation of isotopically labeled bacteria by polymorphonuclear leucocytes and macrophages.J Exp Med. 1963 Jan 1;117:27-42 PMID: 14022146
-
High specific activity iodination of gamma-globulin with iodine-131 monochloride.Cancer Res. 1960 Nov;20:1495-1500 PMID: 13713200
-
Studies on protein uptake by isolated tumor cells. II. Quantitative data on the adsorption and uptake of I-131-serum albumin by Ehrlich ascites tumor cells.J Cell Biol. 1962 Dec;15:437-49 PMID: 13983280
-
Synthetic polypeptide metabolism. 3. Degradation and organ localization of isomeric synthetic polypeptide antigens.J Immunol. 1967 Feb;98(2):236-50 PMID: 6019716
-
THE DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES. MORPHOLOGY, CYTOCHEMISTRY, AND BIOCHEMISTRY.J Exp Med. 1965 Jan 1;121:153-70 PMID: 14253481
-
The in vitro differentiation of mononuclear phagocytes. V. The formation of macrophage lysosomes.J Exp Med. 1966 Apr 1;123(4):757-66 PMID: 5931922
-
Protein uptake in multivesicular bodies in the molt-intermolt cycle of an insect.Science. 1967 Jan 27;155(3761):467-9 PMID: 6015696
-
THE FATE OF DNA-CONTAINING PARTICLES PHAGOCYTIZED BY MAMMALIAN CELLS.J Cell Biol. 1964 Apr;21:105-14 PMID: 14154484
-
The chemistry of iodination.Ann N Y Acad Sci. 1957 Aug 30;70(1):3-18 PMID: 13488250
-
Functions of lysosomes.Annu Rev Physiol. 1966;28:435-92 PMID: 5322983
-
THE IN VITRO DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES. II. THE INFLUENCE OF SERUM ON GRANULE FORMATION, HYDROLASE PRODUCTION, AND PINOCYTOSIS.J Exp Med. 1965 May 1;121:835-48 PMID: 14278233
-
YOLK PROTEIN UPTAKE IN THE OOCYTE OF THE MOSQUITO AEDES AEGYPTI. L.J Cell Biol. 1964 Feb;20:313-32 PMID: 14126875
-
Microkinetospheres and VP satellites of pinocytic cells observed in tissue cultures of Gey's strain HeLa with phase contrast cinematographic techniques.J Biophys Biochem Cytol. 1957 Sep 25;3(5):697-704 PMID: 13475386
-
The utilization of proteins by cultured human cells.J Biol Chem. 1960 Apr;235:1095-7 PMID: 13819086
-
Chromatographic analysis of radioactive iodine compounds from the thyroid gland and body fluids.Methods Biochem Anal. 1954;1:243-64 PMID: 13193531
-
Turnover of rat liver tyrosine transaminase: stabilization after inhibition of protein synthesis.Science. 1967 Apr 28;156(3774):525-8 PMID: 4381474
-
The use of formaldehyde-treated 131-I-albumin in the study of digestive vacuoles and some properties of these particles from mouse liver.J Cell Biol. 1967 Mar;32(3):699-707 PMID: 6034485
-
CYTOCHEMICAL OBSERVATIONS ON THE RELATIONSHIP BETWEEN LYSOSOMES AND PHAGOSOMES IN KIDNEY AND LIVER BY COMBINED STAINING FOR ACID PHOSPHATASE AND INTRAVENOUSLY INJECTED HORSERADISH PEROXIDASE.J Cell Biol. 1964 Mar;20:497-507 PMID: 14128050
-
Protein measurement with the Folin phenol reagent.J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
-
The regulation of pinocytosis in mouse macrophages. I. Metabolic requirements as defined by the use of inhibitors.J Exp Med. 1966 Oct 1;124(4):557-71 PMID: 5950886
-
The in vitro differentiation of mononuclear phagocytes. 3. The reversibility of granule and hydrolytic enzyme formation and the turnover of granule constituents.J Exp Med. 1965 Sep 1;122(3):455-66 PMID: 5320303
-
The release of labeled amino acids from the proteins of rat liver slices.J Biol Chem. 1953 Mar;201(1):143-54 PMID: 13044783
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
